In-Cell NMR and EPR studies of electron transport in Acidithiobacillus ferrooxidans
EMSL Project ID
48716
Abstract
The interior of a cell is a crowded and complex environment. In-Cell NMR is an emerging technology that enables the direct interrogation of biological macromolecules in the cellular environment. However, the study of proteins using this technique has been limited to systems where a non-native protein is overexpressed or introduced exogenously. Here we propose the development and application of In-Cell NMR and EPR techniques for the study of the protein rusticyanin in the iron oxidizing bacteria Acidithiobacillus ferroxidans. Rusticyanin is an abundant periplasmic protein that plays a primary role in the redox chain used to oxidize extracellular iron. If successful, our studies will provide unprecedented atomic resolution structural and functional information about a protein in its native environment, as well as provide new insights into the oxidative respiration of these bacteria.
Project Details
Project type
Limited Scope
Start Date
2015-01-10
End Date
2015-03-12
Status
Closed
Released Data Link
Team
Principal Investigator