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Determining Structures of the APOBEC3-HIV-1 Vif-E3 Ligase Complexes


EMSL Project ID
50763

Abstract

The APOBEC3 family of cytosine deaminases are antiviral factors that can restrict the replication of HIV-1. The virus eliminates this threat by encoding a small protein called Vif that hijacks the host E3 ligase complex and targets the APOBEC3s for proteasomal degradation. Remarkably, Vif can interact with several APOBEC3s (APOBEC3C, 3F, 3G, and 3H) and multiple host factors (CBFbeta, CUL5, and ELOB/C) to efficiently degrade the APOBEC3s. Although many mono-domain APOBEC3 structures and a single partial Vif-E3 ligase complex have been determined, the atomic details of the APOBEC3-Vif interface have remained elusive. Our overall goals are to determine structures of multiple APOBEC3-Vif-E3 ligase complexes. The Aims of this proposal are to screen APOBEC3-Vif-E3 ligase complexes using the Talos Arctica to identify suitable candidates for future Titan Krios data collection and structure determination.

Project Details

Start Date
2019-04-22
End Date
2020-02-29
Status
Closed

Team

Principal Investigator

Nadine Shaban
Institution
University of Minnesota

Co-Investigator(s)

Reuben Harris
Institution
University of Minnesota

Team Members

Harry Scott
Institution
Oregon Health & Science University