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The influence of nitrogen and carbon availability on the dominant microbial communities and their metabolites in a coniferous forest soil


EMSL Project ID
50906

Abstract

The proposed metabolomics analysis will supplement already available data which explores the influence of available C and N in forest soil on microbial community composition and organic matter transformation. We propose that metabolomes from the different soil compartments, combined with microbial community data from RNA and DNA templates, we can track the activity of primary producers (dominant trees) as well as C mobilization and efflux associated with microbes as major decomposers. The available microbial community data suggests that there is a strong effect of season on especially the root- and rhizosphere associated fungal communities, and metabolomics data will allow us to test the hypothesis whether these differences are driven by available sugars. The setting of the experiments at two boundaries of nitrogen-deposition (low in Central Europe, high in Scandinavia) will reveal the dependence of C and N cycling processes and microbiome involved in relation to N content. Microbial community data suggests that C and N-availability is a strong driver of microbial community composition in coniferous soils, and having metabolomes as another layer would add impact to the manuscript being prepared. We expect that the finishing of the proposal will give all missing data for a paper that is under preparation.

Project Details

Project type
Limited Scope
Start Date
2019-06-10
End Date
2019-08-10
Status
Closed

Team

Principal Investigator

Petr Baldrian
Institution
Institute of Microbiology of the CAS

Team Members

Tomas Vetrovsky
Institution
Institute of Microbiology of the CAS

Zander Human
Institution
Institute of Microbiology of the CAS