Identification of plant immune-active metabolic constituents of extracellular bacterial vesicles
EMSL Project ID
51425
Abstract
Cells release vesicles to carry out a variety of functions, including both beneficial and antagonistic inter-kingdom communication. Bacteria release vesicles constitutively and by activation, and their molecular composition has been determined to be critical to their function. Vesicle mediators governing the bacterial interactions with plant hosts remains poorly understood. Our research has shown that bacterial vesicles elicit protective immune responses in plants and this phenomenon is protein independent. To reveal the immune active molecule(s), we propose characterizing the vesicle metabolome. We hypothesize that specific molecules carried by vesicles produced under particular conditions impact plant immune signaling pathways and their protective effects. Previously, we found that the bacterial source of the vesicles and the conditions in which they were produced impact how vesicles activate plant immune responses. For this metabolomic analysis, we propose comparing vesicle samples from a bacterial plant pathogen and non pathogenic strains grown in different types of media. This analysis will reveal key mediators of communication across kingdoms and provide a basis for probing systems level plant-microbe interactions.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2020-10-01
End Date
2022-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members