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Structural analysis of the sole surface glycoprotein of Machupo virus


EMSL Project ID
51579

Abstract

Machupo virus (MACV) was discovered in the 1960s and has remained endemic to Bolivia, causing several sporadic outbreaks of the highly lethal human disease, Bolivian Hemorrhagic Fever (BHF). No vaccines are approved for protection against BHF. Towards the development of a candidate surface glycoprotein (GP)-based vaccine and neutralizing antibodies, and for a more detailed understanding of the antigenic determinants of MACV, we have taken an approach based in single particle analysis using cryo-EM to determine the structure of the MACV GP alone and with bound antibodies. We have produced the single-chain variable fragment (scFv) of the only well-characterized human anti-MACV GP antibody, CR1-07, in complex with the MACV GP ectodomain and have prepared grids of this complex, as well as of the GP alone, for structure determination using single particle analysis and cryo-EM.

Project Details

Start Date
2020-07-15
End Date
2020-10-15
Status
Closed

Team

Principal Investigator

Kathryn Hastie
Institution
La Jolla Institute for Immunology

Team Members

Emilia Arturo
Institution
La Jolla Institute for Immunology