Investigating the Functional Relevance of Glycosylation on Cellulase Enzymes for Biological Deconstruction of Plant Biomass
EMSL Project ID
60678
Abstract
The overall purpose is to use the GH7 enzyme family as model for understanding cellulase molecular function, by investigating how they work as molecular nanomachines at the solid-liquid interface to degrade recalcitrant cellulosic materials. In this project we focus on the role of glycosylation on cellulase structure and activity. We will use mass spectrometry-based proteomics to define the exact glycosylation states of three model cellulases to define the roles do post-translational modifications (PTMs; e.g. glycosylation), on catalytic domain, CBM and linker, play in enzyme-substrate interactions for different types of enzymes. In parallel we will study the interaction between cellulase and substrate using in-situ infrared and atomic force spectroscopy. The data collected here will be the foundation for studying a larger variety of cellulases and dissect critical structural features to improve the efficacy of such enzymes in bioenergy applications.
Project Details
Project type
Limited Scope
Start Date
2023-02-20
End Date
2023-06-30
Status
Closed
Released Data Link
Team
Principal Investigator