Structural and biochemical characterization of glycosyltransferase 47 family proteins from Spirodela to enable predictive biology
EMSL Project ID
60868
Abstract
Complex carbohydrates comprise the majority of plant biomass and are assembled by large families of glycosyltransferases (GTs) that transfer sugars from activated donors to a wide range of acceptor molecules. The GT47 family of proteins, specifically, are involved in almost every aspect of cell wall synthesis, recognizing a variety of saccharide donors and polysaccharide acceptors. This proposal seeks to comprehensively study the GT47 proteins found in the duckweed Spirodela polyrhiza to create a database that can be used to predict GT47 functionality in other organisms. We will use a combination of plant engineering, mass spectrometry, ssNMR, and structural biology to analyze the protein interaction networks of all GT family members in and the plant cell wall structure of S. polyrhiza. Ultimately, the data generated from this proposal will be used to create designer plants, with specified cell wall structures for bioproduction.
Project Details
Project type
FICUS Research
Start Date
2023-10-01
End Date
N/A
Status
Active
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members