Projects

Why is Arctic tundra soil respiration carbon limited at low temperatures?

Lead Institution

University of Toledo

Principal Investigator

Michael Weintraub

Project type

Large-Scale EMSL Research

Soil microbial communities are active during much of the long Arctic winter, despite low temperatures, so overwinter microbial activity is important to global carbon (C) budgets. C-rich Arctic soils…

Dissecting Fe Driven Changes to Metabolism in Photosynthetic Eukaryotes

Lead Institution

Michigan State University

Principal Investigator

Stefan Schmollinger

Project type

Large-Scale EMSL Research

Iron (Fe) is an essential micronutrient for nearly all forms of life, because of its vital role in respiration, photosynthesis, central carbon metabolism, as well as other reactions involving…

Understanding the Roles of Phase State and Phase Separation in the Growth of Secondary Organic Aerosols

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Rahul Zaveri

Project type

Large-Scale EMSL Research

We propose to investigate the fundamental roles of phase state and phase separation in the growth of secondary organic aerosols (SOA) formed from oxidation of selected volatile organic compounds (in…

Influence of hematite crystal face on organic ligand surface interactions with iron oxide/water interfaces studied using nonlinear spectroscopy techniques and molecular simulation methods.

Lead Institution

University of Puget Sound

Principal Investigator

Amanda Mifflin

Project type

Large-Scale EMSL Research

The proposed work will examine the interfacial phenomena of the organic ligand acetohydroxamic acid (aHa) at the hematite/water interface and the molecular structure at organic/aqueous interface…

Identification of plant immune-active metabolic constituents of extracellular bacterial vesicles

Lead Institution

Duke University

Principal Investigator

Meta Kuehn

Project type

Large-Scale EMSL Research

Cells release vesicles to carry out a variety of functions, including both beneficial and antagonistic inter-kingdom communication. Bacteria release vesicles constitutively and by activation, and…

Molecular understanding of nutrient and organic sorption on model mineral surfaces

Lead Institution

Oregon State University

Principal Investigator

Kelsey Stoerzinger

Project type

Large-Scale EMSL Research

This proposal aims to obtain molecular understanding of the sorption of nitrogen (N) oxyanions and model organic species on model mineral surfaces, and ultimately provide better constraints in…

Towards a systems model of circadian control of gene function to regulate metabolic phenotype and cellulase production in Neurospora crassa

Lead Institution

Rensselaer Polytechnic Institute

Principal Investigator

Jennifer Hurley

Project type

Large-Scale EMSL Research

Biofuel production using cellulases extracted from fungi has the potential to be a major resource to replace fossil fuels, and regulating the synthesis of these and other enzymes is crucial for the…

Exploring the native cell wall nanoarchitecture of engineered bioenergy crops via multi-dimensional solid-state NMR.

Lead Institution

University of Adelaide

Principal Investigator

Jennifer Mortimer

Project type

Large-Scale EMSL Research

Carbon dioxide from the atmosphere, an inorganic carbon source, is fixed by plants via photosynthesis to form the polysaccharides and lignin that constitute the plant cell wall. The plant cell wall,…

Selective Sensors for High Spatiotemporal Imaging of Small Molecule Targets in Metabolic Pathways of Plants and Cells involved in Bio-energy Processes

Lead Institution

University of Texas at Austin

Principal Investigator

Yi Lu

Project type

Large-Scale EMSL Research

As a member of the Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), a DOE Bioenergy Center at the University of Illinois at Urbana-Champaign, we aim to develop selective sensors to…

Cryo-FIB and High-Vacuum Cryo-Transfer for Atom Probe Tomography Analysis of Organic Molecular Materials

Lead Institution

Colorado School of Mines

Principal Investigator

Jeramy Zimmerman

Project type

Large-Scale EMSL Research

The morphology of mixed molecular solids impacts macroscopic properties of the material (e.g. conductivity). Our group has developed atom probe tomography (APT) analysis methods for organic small…

Identifying dynamic sources and transformations of dissolved organic matter in coastal watersheds exposed to extreme flooding

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Nicholas Ward

Project type

Large-Scale EMSL Research

The primary objective of this proposal is to improve mechanistic understanding of the hydrobiogeochemical processes governing the cycling of organic matter (OM) across the land-to-sea continuum…

High-resolution imaging of Rhodobacter sphaeroides strains with increased lipid production

Lead Institution

University of Wisconsin, Madison

Principal Investigator

Timothy Donohue

Project type

Large-Scale EMSL Research

Rhodobacter sphaeroides is an alpha-proteobacterium which can remodel its cell envelope under low-oxygen or anaerobic conditions, by increasing its lipid content and forming intracytoplasmic membrane…

The Diurnal Redox Proteome of Chlamydomonas

Lead Institution

Michigan State University

Principal Investigator

Daniela Strenkert

Project type

Large-Scale EMSL Research

Nearly all organisms exhibit diurnal metabolic cycles, especially phototrophs, whose metabolism is strictly dependent on light. Transcriptomics experiments in cyanobacteria, algae and plants have…

Chemical Imaging and Ice Nucleation Properties of Individual Arctic Atmospheric Particles

Lead Institution

University of Michigan

Principal Investigator

Kerri Pratt

Project type

Large-Scale EMSL Research

The proposed project will address critical gaps in our understanding of the Arctic aerosol sources, chemical composition, and ice nucleation properties through chemical imaging and ice nucleation…

Tracking carbon flow through syntrophic interspecies interactions in methanogenic microbiomes

Lead Institution

University of British Columbia

Principal Investigator

Ryan Ziels

Project type

Large-Scale EMSL Research

Determining in situ metabolic processes and interactions among diverse assemblages of microorganisms remains a critical challenge in microbial ecology. Consequently, identifying novel genes and…

Environmental Photochemical Catalysis and Microphysical Properties of Fe-Organic Aerosols

Lead Institution

Purdue University

Principal Investigator

Alexander Laskin

Project type

Large-Scale EMSL Research

The intent of this collaborative research is to investigate photo-catalytic effects of atmospheric aging on the chemical composition and the microphysical properties of secondary organic aerosol (SOA…

Connecting Structure and Function in Anaerobic Fungal Cellulosomes

Lead Institution

University of California, Santa Barbara

Principal Investigator

Michelle O'Malley

Project type

Large-Scale EMSL Research

This proposal seeks use of EMSL resources to characterize the unique, multi-enzyme cellulosomes made by anaerobic gut fungi that are found within large herbivores. Cellulosomes enable anaerobic…

Uncovering the phosphoproteome of arbuscular mycorrhizal roots

Lead Institution

Boyce Thompson Institute for Plant Research

Principal Investigator

Maria Harrison

Project type

Large-Scale EMSL Research

During the endosymbiotic association of plants and arbuscular mycorrhizal (AM) fungi, the fungus inhabits the root cortical cells where it delivers mineral nutrients to its plant host, and in return…

Modeling Solvent Effects in Mixed-Solvent Environments for Improved Biomass Conversion

Lead Institution

University of Wisconsin, Madison

Principal Investigator

Reid Van Lehn

Project type

Large-Scale EMSL Research

The conversion of lignocellulosic biomass is a promising method to produce biofuels and chemicals for further microbial conversion from a renewable carbon resource via liquid-phase, acid-catalyzed…

Integrated experimental and modeling study to investigate vertical profile of atmospheric particles via tethered balloon system

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Fan Mei

Project type

Large-Scale EMSL Research

We propose a combined experimental and modeling study to characterize the vertical profile of atmospheric aerosol collected via a tethered balloon system using multi-modal micro-spectroscopy…