Projects

Characterizing the Composition, Morphology and Reactivity of Bacterial Nanowires using Advanced Scanning Probe Microscopy, MS-Based Proteomics and Scanning Electron Microscopy

Lead Institution

J. Craig Venter Institute

Principal Investigator

Yuri Gorby

Project type

Large-Scale EMSL Research

This project seeks to investigate the composition, morphology and reactivity of electrically conductive appendages known as bacterial nanowires. The research will capitalize on numerous analytical,…

Modeling Multiscale-Multiphase-Multicomponent Subsurface Reactive Flows using Advanced Computing

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Glenn Hammond

Project type

Large-Scale EMSL Research

During the course of the project we will apply PFLOTRAN to uranium migration at the Hanford 300 Area and to CO2 sequestration in subsurface geologic formations. In addition, we will test new…

Conformational Dynamics of Pin1 Regulation of APP processing and Abeta Production

Lead Institution

Cornell University

Principal Investigator

Linda Nicholson

Project type

Large-Scale EMSL Research

The proposed studies fall within the EMSL science theme of "Biological Interactions and Dynamics", specifically in the effort to understand how the "readout", or experimentally…

Mechanistic Studies of Protein Allostery at the Atomistic Scale

Lead Institution

University of California, Berkeley

Principal Investigator

Jhih-Wei Chu

Project type

Large-Scale EMSL Research

Allostery refers to the change of protein conformation in response to ligand binding, biochemical modifications, or interactions with other proteins. In this proposal, we plan to study protein…

STRUCTURAL STUDIES OF REGULATORS OF HISTONE PROTEIN SYNTHESIS

Lead Institution

University of North Carolina

Principal Investigator

Roopa Thapar

Project type

Large-Scale EMSL Research

STRUCTURAL STUDIES OF REGULATORS OF HISTONE PROTEIN SYNTHESIS. Roopa Thapar and William F. Marzluff In each cell cycle a eukaryotic cell must not only replicate its DNA, but must also…

The Aerosol Modeling Testbed

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jerome Fast

Project type

Large-Scale EMSL Research

An Aerosol Modeling Testbed will be developed that will simplify and greatly increase the efficiency of evaluating new aerosol treatments for regional and global climate models. The Aerosol Modeling…

Coupled Hydrological and Geochemical Studies on Uranium Plumes at Hanford Site: Reactions, Spatial Distributions, Speciation, and Future Mobility

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Zheming Wang

Project type

Large-Scale EMSL Research

Massive quantities (exceeding 85 tons) of U currently reside in the Hanford vadose zone, and will threaten groundwater and the accessible environment for generations to come. Present insufficient…

Interfacial Phenomena in Cu Plating

Lead Institution

eMAT Technology LLC

Principal Investigator

Valery Dubin

Project type

Large-Scale EMSL Research

Electroplated Cu on a physical vapor deposited (PVD) Cu seed layer with a PVD Ta-based barrier is now an integrated process for the production of advanced interconnects for integrated circuits (IC),…

Mechanisms of Ubiquitin Transfer and Regulation

Lead Institution

University of Washington

Principal Investigator

Rachel Klevit

Project type

Large-Scale EMSL Research

Protein ubiquitination is a powerful regulatory process that influences nearly every aspect of eukaryotic cell biology. Pathways ranging from cell-cycle progression and differentiation to DNA repair…

Exploring the Proteome of E. coli Following Adaptation to Environmental and Genetic Perturbation

Lead Institution

University of California, San Diego

Principal Investigator

Bernhard Palsson

Project type

Large-Scale EMSL Research

Microorganisms rapidly adapt to changes in the environment which ensures their survival under suboptimal conditions. It is well known that microorganisms display genomic plasticity that enables…

Experimental and Theoretical Investigations on the
Chemistry and Dynamics of Ammonia Borane Dehydrogenation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Autrey

Project type

Large-Scale EMSL Research

Increasing demands for clean energy sources that do not add CO2 and other pollutants to the environment have resulted in increased worldwide interest in the possibilities of a "hydrogen economy…

Determination of the CCR5 and CXCR4 Antagonist and Agonist Binding Site.

Lead Institution

Montana State University

Principal Investigator

Edward Dratz

Project type

Large-Scale EMSL Research

This proposal focuses on determining the locations of the agonist and antagonist binding pockets for the 7-transmembrane spanning G-Protein coupled receptors (GPCR) CCR5 and CXCR4. G-protein coupled…

Proteomic-based mechanistic analysis of components involved in the immunoprotective pathways regulated by low-dose radiation and the medicinal mushroom Trametes versicolor

Lead Institution

Bastyr University

Principal Investigator

Cynthia Wenner

Project type

Large-Scale EMSL Research

The goal of this proposal is to identify molecular mechanisms that underlie radio-adaptive immune responses. We propose to use quantitative mass spectrometry to investigate the effects of…

Doping inorganic wide-bandgap semiconductors for future energy devices

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weilin Jiang

Project type

Large-Scale EMSL Research

Solid-state lighting (SSL) has a significantly higher efficiency of converting electricity to visible light compared to the incandescent (5%) or fluorescent (20%) lighting due to elimination of…

Coprecipitation of Aluminum Hydroxides and Calcium Carbonates during Denitrification: A Stabilization Strategy for Oxidized Metals and Radionuclides

Lead Institution

Oregon State University

Principal Investigator

Jonathan Istok

Project type

Large-Scale EMSL Research

Research conducted at the Field Research Center (FRC) at Oak Ridge National Laboratory and at the Old Rifle site has demonstrated that it is possible to stimulate indigenous microbial activity and…

Validation of Whole-Core Determination of Soil Hydraulic Properties

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Michael Fayer

Project type

Large-Scale EMSL Research

The EMSL Subsurface Flow and Transport Laboratory developed a fully automated Hydraulic Property Apparatus (HPA) that is a significant advance in measurement technology. The HPA uses the multistep…

Measurement and Optimization of the Redox Properties of Enzymes Using Single-Molecule Imaging, Controlled Electrochemical Potential, and Nanoscale Confinement

Lead Institution

Western University of Health Sciences

Principal Investigator

Thomas Squier

Project type

Large-Scale EMSL Research

We propose a new multi-disciplinary approach to single-molecule spectroscopy to image the redox kinetics of enzymes in spatially confined geometries under controlled electrochemical potential. We…

Thermodynamic and Chemical Properties of La1-xSrxCoxFe1-xO3 from First-Principles Calculations: Implications for Use as SOFC Cathodes

Lead Institution

University of Florida

Principal Investigator

Susan Sinnott

Project type

Large-Scale EMSL Research

The objective of the proposed research is to obtain an atomic-level understanding of heterogeneous electrocatalytic phenomena at the surface of mixed conducting solid oxide fuel cell (SOFC) cathodes…

Ground squirrel proteome: protein modifications through torpor and arousal

Lead Institution

University of Colorado

Principal Investigator

Lois Epperson

Project type

Large-Scale EMSL Research

Liver samples from the hibernator, golden-mantled ground squirrel, when separated by 2D gel electrophoresis, demonstrate a quantifiable and as yet undefined physical modification that manifests…

Comparative Proteomic Analysis of Naturally Occurring Anaerobic Methane Oxidizing Communities

Lead Institution

University of British Columbia

Principal Investigator

Steven Hallam

Project type

Large-Scale EMSL Research

Methane is a potent greenhouse gas whose atmospheric concentration has increased significantly due to anthropogenic activities and fluctuated naturally over glacial and interglacial cycles. While…