Projects

High-resolution imaging of Rhodobacter sphaeroides strains with increased lipid production

Lead Institution

University of Wisconsin, Madison

Principal Investigator

Timothy Donohue

Project type

Large-Scale EMSL Research

Rhodobacter sphaeroides is an alpha-proteobacterium which can remodel its cell envelope under low-oxygen or anaerobic conditions, by increasing its lipid content and forming intracytoplasmic membrane…

The Diurnal Redox Proteome of Chlamydomonas

Lead Institution

Michigan State University

Principal Investigator

Daniela Strenkert

Project type

Large-Scale EMSL Research

Nearly all organisms exhibit diurnal metabolic cycles, especially phototrophs, whose metabolism is strictly dependent on light. Transcriptomics experiments in cyanobacteria, algae and plants have…

Chemical Imaging and Ice Nucleation Properties of Individual Arctic Atmospheric Particles

Lead Institution

University of Michigan

Principal Investigator

Kerri Pratt

Project type

Large-Scale EMSL Research

The proposed project will address critical gaps in our understanding of the Arctic aerosol sources, chemical composition, and ice nucleation properties through chemical imaging and ice nucleation…

Tracking carbon flow through syntrophic interspecies interactions in methanogenic microbiomes

Lead Institution

University of British Columbia

Principal Investigator

Ryan Ziels

Project type

Large-Scale EMSL Research

Determining in situ metabolic processes and interactions among diverse assemblages of microorganisms remains a critical challenge in microbial ecology. Consequently, identifying novel genes and…

Environmental Photochemical Catalysis and Microphysical Properties of Fe-Organic Aerosols

Lead Institution

Purdue University

Principal Investigator

Alexander Laskin

Project type

Large-Scale EMSL Research

The intent of this collaborative research is to investigate photo-catalytic effects of atmospheric aging on the chemical composition and the microphysical properties of secondary organic aerosol (SOA…

Connecting Structure and Function in Anaerobic Fungal Cellulosomes

Lead Institution

University of California, Santa Barbara

Principal Investigator

Michelle O'Malley

Project type

Large-Scale EMSL Research

This proposal seeks use of EMSL resources to characterize the unique, multi-enzyme cellulosomes made by anaerobic gut fungi that are found within large herbivores. Cellulosomes enable anaerobic…

Uncovering the phosphoproteome of arbuscular mycorrhizal roots

Lead Institution

Boyce Thompson Institute for Plant Research

Principal Investigator

Maria Harrison

Project type

Large-Scale EMSL Research

During the endosymbiotic association of plants and arbuscular mycorrhizal (AM) fungi, the fungus inhabits the root cortical cells where it delivers mineral nutrients to its plant host, and in return…

Modeling Solvent Effects in Mixed-Solvent Environments for Improved Biomass Conversion

Lead Institution

University of Wisconsin, Madison

Principal Investigator

Reid Van Lehn

Project type

Large-Scale EMSL Research

The conversion of lignocellulosic biomass is a promising method to produce biofuels and chemicals for further microbial conversion from a renewable carbon resource via liquid-phase, acid-catalyzed…

Integrated experimental and modeling study to investigate vertical profile of atmospheric particles via tethered balloon system

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Fan Mei

Project type

Large-Scale EMSL Research

We propose a combined experimental and modeling study to characterize the vertical profile of atmospheric aerosol collected via a tethered balloon system using multi-modal micro-spectroscopy…

Probing Iron Oxide Mineral Aggregation Behavior with Soil Organic Matters

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Xin Zhang

Project type

Large-Scale EMSL Research

The goal of this project is to develop a fundamental understanding of iron oxide nanoparticle (NPs) aggregation mechanisms with soil organic matters (SOM). Heteroaggregation is a ubiquitous but…

Deciphering controls on metal migration within floodplains: The critical role of redox environments on metal-organic complexes

Lead Institution

Stanford University

Principal Investigator

Scott Fendorf

Project type

Large-Scale EMSL Research

Dissolved organic matter (DOM) is a major but poorly understood control over the mobility of micronutrient and contaminant metals in surface and subsurface systems. Variations in the chemical…

Sticky Roots -- Implications of Altered Rhizodeposition for the Fate of Mineral-associated Organic Matter in Natural Ecosystems

Lead Institution

University of Massachusetts Amherst

Principal Investigator

Marco Keiluweit

Project type

Large-Scale EMSL Research

The proposed research aims to improve mechanistic understanding of links among rhizodeposition of reactive organic compounds by plant roots, microbial activity, and the stability of mineral…

Carbon signaling cascades that enable the carbohydrate-selective plant biomass degradation by brown rot fungi

Lead Institution

University of Minnesota

Principal Investigator

Jiwei Zhang

Project type

Large-Scale EMSL Research

Fungi are dominant microbes for carbon turnover of plant biomass in terrestrial systems. Some of them, namely brown rot fungi, evolved more efficient mechanisms than others, causing the fast…

Mineralogy and Soil Organic Matter Composition as Drivers of Reactive Nitrogen Emissions from Midwestern Hardwood Forest Soils

Lead Institution

Indiana University - Bloomington

Principal Investigator

Jonathan Raff

Project type

Exploratory Research

The overall goal of this proposal is to investigate how plant-microbe-mineral interactions drive emissions of reactive nitrogen from soils. Fluxes of reactive nitrogen oxides [NOy = NO + NO2 + HONO]…

Quantifying Peptide Abundance Ratios

Lead Institution

North Carolina State University

Principal Investigator

Michael Goshe

Project type

Exploratory Research

Regulation of protein expression and modulation of post-translational modifications (PTMs) are biochemical processes that are vital to cellular function. Phosphorylation and glycosylation are two of…

3D Flow and transport in highly heterogeneous porous media

Lead Institution

Oregon State University

Principal Investigator

Brian Wood

Project type

Exploratory Research

We will be examining flow and transport, specifically macrodispersion, in highly heterogeneous media in 3-dimensions. Particular focus will be on tailing phenomena that lead to non-Fickian spreading…

Multiple Sensor Data Integration (PNNL Scope # 90001)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Nancy Valentine

Project type

Exploratory Research

Development of a mathematical/statistical framework for nuclear, chemical, and biological (NCB) sensor integration and decision analysis is proposed. In particular, a statistical model for…

High-Field Dynamic Nuclear Polarization Technique for Probing the Cellular Structure of Polysaccharides and Lignin in Plants

Lead Institution

Michigan State University

Principal Investigator

Tuo Wang

Project type

Exploratory Research

The NMR Facility at EMSL is going to house the first commercial high-field (800 MHz/527 GHz) Dynamic Nuclear Polarization (DNP) system in the United States. This state-of-art instrument will allow us…

Evaluating viral activity and infection dynamics during wet-up of dry grassland soils

Lead Institution

University of California, Davis

Principal Investigator

Joanne Emerson

Project type

Exploratory Research

After months of dryness, the first heavy rainfall in Mediterranean climates drives a predictable pulse of CO2 emissions and soil microbial activity each year. Responsive microbial populations begin…

Controls on the Composition of Persistent Microbial-derived Organic Matter in Soil

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kirsten Hofmockel

Project type

Exploratory Research

Current estimates suggest that soils contain 1.5 x 10^12 tons of carbon, or roughly three times more carbon than the atmosphere, and represent a giant reservoir for potential greenhouse gas emission…