Amy Sims

Integrative Multi-PTM Profiling by an Automated Shotgun Proteomics Coupled with Top-down Proteomics

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tong Zhang

Project type

Contracted Time

To use PTM data to understand cell signaling and profile molecular phenotypes, PNNL has made substantial strides in advancing phosphoproteome and thiol redox proteome profiling. However, the current…

Targeted Nanobody Generation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Samantha Powell

Project type

Contracted Time

Here we introduce a nanobody screening pipeline for the generation of target specific nanobodies. Nanobodies will be screened against a synthetic yeast library and then undergo alternating rounds of…

Integrative Multi-PTM Profiling by an Automated Shotgun Proteomics Coupled with Top-down Proteomics

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tong Zhang

To use PTM data to understand cell signaling and profile molecular phenotypes, PNNL has made substantial strides in advancing phosphoproteome and thiol redox proteome profiling. However, the current…

COVID-19: Identifying Metabolite Biomarkers in Human Coronavirus infected Primary Human Lung Cultures

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Amy Sims

Project type

Limited Scope

Severe acute respiratory syndrome coronavirus (SARS-CoV 2) is the causative agent of coronavirus infectious disease 2019 (COVID-19) and is responsible for the first recorded human coronavirus…

Manipulation of Host Signature Expression Following Respiratory Virus Infection

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

Pathogen invasion of host cells causes a myriad of functional changes including alterations of chromatin accessibility often limiting defense responses, shunting of cellular resources to centers of…

EMSL Science Overview

Amy Sims

Projects

Integrative Multi-PTM Profiling by an Automated Shotgun Proteomics Coupled with Top-down Proteomics

Targeted Nanobody Generation

Integrative Multi-PTM Profiling by an Automated Shotgun Proteomics Coupled with Top-down Proteomics

COVID-19: Identifying Metabolite Biomarkers in Human Coronavirus infected Primary Human Lung Cultures

Manipulation of Host Signature Expression Following Respiratory Virus Infection