Johannes Scholten

PNNL Metabolomics Measurement and Validation Development for Renewable Energy Research

Lead Institution

Washington State University

Principal Investigator

Paul Majors

Project type

Large-Scale EMSL Research

This capability-development project will provide PNNL and DOE with a unique live / in-situ metabolomics capability for microbial bioenergy development. The technology will be applicable for the…

Application of High-Throughput Proteomics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mary Lipton

The understanding of how cells function at the systems level will greatly benefit from the development of new approaches capable of making global measurements of protein expression (proteome). The…

Integrated NMR/SIP technologies for the functional and structural characterization of microbial communities

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Johannes Scholten

Project type

Large-Scale EMSL Research

We propose to combine stable isotope probing (SIP) of DNA (SIP-DNA) with real-time on-line SIP-nuclear magnetic resonance (SIP-NMR) analysis of microbial community metabolism. SIP-NMR provides access…

Proteomics of Bioenergetics (PNNL SCOPE # 90001 , LDRD)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Johannes Scholten

Project type

Exploratory Research

HPLC analysis and quanitation of organic acids is a critical tool to track metabolic changes during growth in microbial cultures. Because are own HPLC system has ended it's useful life, Tom…

Interrogation of Glucose Metabolism by Oral Biofilms Using Combined NMR/Optical Spectroscopy and Stable Isotope Labeling

Lead Institution

Washington State University

Principal Investigator

Paul Majors

Project type

Capability Research

Dental caries disease and associated tooth decay (demineralization) is correlated with a decrease in pH and increase in the proportion of acidogenic and aciduric species within the attached microbial…

Interrogation of Glucose Metabolism by Oral Biofilms Using Combined NMR/Optical Spectroscopy and Stable Isotope Labeling

Lead Institution

Washington State University

Principal Investigator

Paul Majors

The overall project goal is to develop a robust technology capable of characterizing overall community metabolic function and the roles of individual species in mixed-community biofilms. This effort…

PNNL Metabolomics Measurement and Validation Development for Renewable Energy Research

Lead Institution

Washington State University

Principal Investigator

Paul Majors

Project type

Capability Research

We propose the in-situ/live analysis of microbes inside of a controlled bioreactor. Further, we will determine the effects of existing extraction methods by comparing quantitative NMR metabolite…

EMSL Science Overview

Johannes Scholten

Projects

PNNL Metabolomics Measurement and Validation Development for Renewable Energy Research

Application of High-Throughput Proteomics

Integrated NMR/SIP technologies for the functional and structural characterization of microbial communities

Proteomics of Bioenergetics (PNNL SCOPE # 90001 , LDRD)

Interrogation of Glucose Metabolism by Oral Biofilms Using Combined NMR/Optical Spectroscopy and Stable Isotope Labeling

Interrogation of Glucose Metabolism by Oral Biofilms Using Combined NMR/Optical Spectroscopy and Stable Isotope Labeling

PNNL Metabolomics Measurement and Validation Development for Renewable Energy Research