Madelyn Berger

The molecular basis for the role of apolipoprotein A-II in cholesterol and triglyceride metabolism

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Melchior

Project type

Cryo-EM Only

Apolipoprotein (apo)A-II is an abundant human plasma protein primarily in high-density lipoproteins (HDL) but also in very low density lipoproteins (VLDL) and chylomicrons. Despite a large literature…

Pentosan Polysulfate Sodium Profiling with Mass Spectrometry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Cort

PPS is a semisynthetic heterogeneous sulfated polysaccharide derived from xylan, the β-1,4-linked polymer of xylose that is the backbone structural component of hemicellulose in most types of wood…

Predicting adverse biological effects for chemicals of concern through integration of functional profiling with artificial intelligence/machine learning (AI/ML)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vivian Lin

We will use thermal proteome profiling (TPP) to identify protein targets and pathways affected by exposure to a chemical of concern (COC). As a next step, we will use an artificial intelligence…

Pentosan Polysulfate Sodium Profiling with Mass Spectrometry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Cort

PPS is a semisynthetic heterogeneous sulfated polysaccharide derived from xylan, the β-1,4-linked polymer of xylose that is the backbone structural component of hemicellulose in most types of wood…

The molecular basis for the role of apolipoprotein A-II in cholesterol and triglyceride metabolism

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Melchior

Apolipoprotein (apo)A-II is an abundant human plasma protein primarily in high-density lipoproteins (HDL) but also in very low density lipoproteins (VLDL) and chylomicrons. Despite a large literature…

Structural profiling of the molecular interactome to define phenotype

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Melchior

The functional pathways that define phenotype are reflected by protein quinary structure; the transient encounters of proteins with other proteins, molecules, cofactors, and nucleic acids that…

Detergent-free extraction of membrane proteins and lipids from native cells

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Melchior

Membrane proteins account for >30% of most genomes, yet only make up <4% of reported high-resolution structures in the protein data bank. Current approaches for isolating membrane proteins from…

Integrated multi-modal approach for structure and function determination of lignin-forming protein complexes isolated from plants

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Cort

We propose to apply an integrated multi-modal structural biology approach to investigate a biosynthetic protein complex in plants that is hypothesized to organize and guide lignin biosynthesis via…

Impact of apolipoprotein E isoforms on brain lipoproteins

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Melchior

Cerebrovascular dysfunction leads to the deposition of amyloid plaques and neurofibrillary tangles in the brain which underlie the development of Alzheimer’s disease (AD); an epidemic that…

EMSL Science Overview

Madelyn Berger

Projects

The molecular basis for the role of apolipoprotein A-II in cholesterol and triglyceride metabolism

Pentosan Polysulfate Sodium Profiling with Mass Spectrometry

Predicting adverse biological effects for chemicals of concern through integration of functional profiling with artificial intelligence/machine learning (AI/ML)

Pentosan Polysulfate Sodium Profiling with Mass Spectrometry

The molecular basis for the role of apolipoprotein A-II in cholesterol and triglyceride metabolism

Structural profiling of the molecular interactome to define phenotype

Detergent-free extraction of membrane proteins and lipids from native cells

Integrated multi-modal approach for structure and function determination of lignin-forming protein complexes isolated from plants

Impact of apolipoprotein E isoforms on brain lipoproteins