High resolution Matrix assisted Laser Desorption/Ionization imaging of biological samples
EMSL Project ID
3171
Abstract
The use of matrix assisted laser desorption/ionization in protein identification tremendously in the last decade. This approach has been used to perform "protein imaging" of biological samples to provide critical spatial distribution information within those samples. However this approachhas suffered from limitations due to sample complexity (and therefore competitive ionization effects for larger proteins) and inadequate spatial resolution to examine microbial cells and structures. Recent work in collaboration with EMSL staff member Kenneth Beck has resulted in an instrumental modification that allows for greater spatial resolution for performing MALDI and LDI imaging of biological samples. Furthermore, this research has been aimed at examining low molecular weight compounds that are more challenging to image by traditional optical methods and will not suffer the same loss of sensitivity
experienced for larger biomolecules. A spatial resolution of < 500 nm has been achieved while retaining the ability to analyze peptide and small molecule samples. The instrumental limitations imposed by the current setup hinder further development of this approach.
Specifically, the ability to raster across the sample (necessary for imaging) and precisely position the necessary optics is limited. The aim of this project is to perform further modification as well as adapt this approach to additional instrumentation. Furthermore, this approach can be adapted to mass spectrometers with atmospheric interfaces to eliminate the need for sample preparations compatible with high vacuum and provide space for high resolution positioning devices. This will result in a more flexible capability that will be able to produce truly high-resolution chemical/molecular image data on complex biological
samples.
Project Details
Project type
Exploratory Research
Start Date
2002-12-10
End Date
2005-01-06
Status
Closed
Released Data Link
Team
Principal Investigator
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