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Preliminary Proteomics of Microbial-Host Systems


EMSL Project ID
33214

Abstract

In this collaborative study we will analyze and characterize microbial-host interactions using proteomics. The serum of healthy and anthrax-infected macaques will be analyzed to assess qualitatively the influence of the microbe on host serum protein homeostasis, and the relative abundance of microbe proteins in the sera. This project is designed to perform proof of concept experiments that are specifically oriented around discovering potential host-pathogen interactions. This study will also be used to develop an accurate mass and time tag archive for macaque serum.
The succesful completion of this work may elucidate microbe proteins and host-microbe protein interactions critical to the pathogenesis of the microbe. Anthrax has had considerable media attention in recent years, and is infamous for its facile host invasion and rapid pathogenesis. This study may elucidate some of the biology related to anthrax infection. This work requires the EMSL facilities for its high-throughput mass spectrometry facilities and proteomics sample preparation laboratory.

Project Details

Start Date
2009-03-04
End Date
2012-03-04
Status
Closed

Team

Principal Investigator

Joshua Adkins
Institution
Pacific Northwest National Laboratory

Team Members

Brooke Kaiser
Institution
Pacific Northwest National Laboratory

Aaron Wright
Institution
Pacific Northwest National Laboratory