Spatially Resolved Proteomic studies of Brains Obtained from Control Mice and Treated Mice Simulating Parkinson's Disease
EMSL Project ID
3567b
Abstract
Research on the three-dimensional transcriptome of voxelated mice brains has demonstrated that significant RNA expression differences are detectable when comparing the brain transcriptomes of control mice to mice that have received elevated doses of methamphetamine as a model for Parkinson?s Disease. It is desirable to apply a similar three-dimensional proteomic approach on mouse brain voxels.Initially, brains and dissected brains (specifically dissected striatum) from untreated, control mice and methamphetamine treated mice will be used to develop protein extraction methods from brain tissue. These protein lysates will subsequently be used for the initial development of an accurate mass and time (AMT) tag database for characterizing the brain proteomes from treated and untreated mice.
The development of both the brain tissue protein extraction method and the AMT tag database will eventually be extended to mice brains that are three-dimensionally sectioned into voxels for obtaining the 3-D proteomic map of mouse brain tissue. This 3-D proteomic map will then be overlaid on the 3-D map of the mouse brain transcriptome providing the first display of this type.
Project Details
Start Date
2007-12-10
End Date
2010-12-12
Status
Closed
Released Data Link
Team
Principal Investigator