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Rapid quantification of fecal microbiome-associated enzyme functional activities for microtiter plate-based probe assays


EMSL Project ID
50138

Abstract

Characterizing the role of gut microbiota in human drug metabolism will be an important step towards tailored/personalized therapy that would be more efficient, cost-effective, and with lower adverse drug events. Phylogenetic analyses have increased our knowledge of the composition of the human gut microbiome, but we do not yet fully understand the mechanisms by which microbes impact host health. Glucuronidase enzymes contribute to enterohepatic recycling of therapeutic drug compounds, resulting in impaired or prolonged drug activity. We propose the development and application of a microtiter plate-based chemical probe assay that can facilitate the rapid quantification of fecal microbiome-associated enzyme functional activities (“gut pharmacomicrobiomics”).

Project Details

Start Date
2018-01-22
End Date
2018-09-30
Status
Closed

Team

Principal Investigator

Aaron Wright
Institution
Pacific Northwest National Laboratory

Team Members

Kristoffer Brandvold
Institution
Pacific Northwest National Laboratory