Rapid quantification of fecal microbiome-associated enzyme functional activities for microtiter plate-based probe assays
EMSL Project ID
50138
Abstract
Characterizing the role of gut microbiota in human drug metabolism will be an important step towards tailored/personalized therapy that would be more efficient, cost-effective, and with lower adverse drug events. Phylogenetic analyses have increased our knowledge of the composition of the human gut microbiome, but we do not yet fully understand the mechanisms by which microbes impact host health. Glucuronidase enzymes contribute to enterohepatic recycling of therapeutic drug compounds, resulting in impaired or prolonged drug activity. We propose the development and application of a microtiter plate-based chemical probe assay that can facilitate the rapid quantification of fecal microbiome-associated enzyme functional activities (“gut pharmacomicrobiomics”).
Project Details
Start Date
2018-01-22
End Date
2018-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members