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Proteomics Identification Characterization of Biological Agents and Toxins


EMSL Project ID
50693

Abstract

Biological toxins are of human health and biosecurity concern. Identification of such toxins often relies on enzyme-linked immunosorbent assay (ELISA) methods, but orthogonal approaches are desired. The objective of this project is to develop mass spectrometry-based assays to identify and characterize protein and peptide toxins. The potential impact of this research is wide-reaching, including application in the public health, law enforcement and national security fields. Utilization of EMSL resources, specifically the Q-Exactive HF instrument, will be essential for generation of high quality data for use in downstream data analysis applications and to align with end-user instrumentation. Since toxins will either be analyzed in inactive forms or at low levels, no significant toxicity risks are anticipated.

Project Details

Start Date
2019-04-01
End Date
2021-09-30
Status
Closed

Team

Principal Investigator

Eric Merkley
Institution
Pacific Northwest National Laboratory