Structural characterization of the Helicobacter pylori Cag T4SS using single particle cryo-EM
EMSL Project ID
51298
Abstract
Using cryo-EM we determined the structure of the 5.6 MDa H. pylori Cag T4SS at a resolution of ~3.6 Å. The Hp T4SS is structurally different from T4SS structures seen in other species and has an unexpected symmetry mismatch between the 14-fold outer membrane core complex (OMCC) and a contiguous 17-fold periplasmic ring complex (PRC). There are no crystal structures of the individual T4SS components and the 3.6 Å resolution did not allow us to build the entire model de novo. We are requesting Titan microscope time at the national center because the University of Michigan cryo-EM facility is closed for construction from December 2019 until June 2020. Our goal is to collect the data required to push the resolution of the complex to sub-3.0 Å, allowing us to build models of all the T4SS components. We have grids that are screened and ready for imaging in the Titan Krios.
Project Details
Start Date
2020-03-15
End Date
2021-03-17
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members