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Tom1/HUWE1 and its interaction with Spt6


EMSL Project ID
51352

Abstract

Our lab reported that the transcriptional regulator, Spt6, contains a tandem-SH2 domain that binds RNA pol II through a novel interaction that requires multiple phosphorylation site on the Rpb1 linker (Sdano, 2017). Subsequently, we have discovered that the conserved 374 kDa HECT-domain ubiquitin ligase, Tom1(yeast)/HUWE1(human) also binds Spt6 in a phosphorylation-dependent manner that is competitive with RNA Pol II. Furthermore, our unpublished RNA-seq data indicate that Tom1 has dramatic impact on transcriptional activity. Moreover, others have reported roles for Tom1 in mRNA export from the nucleus, with degradation of transcriptional regulators and histones, and with ribosomal quality control. We are determining the structure of Tom1 alone and in complex with both Spt6 and substrates.

Project Details

Start Date
2020-05-15
End Date
2021-03-17
Status
Closed

Team

Principal Investigator

Christopher Hill
Institution
University of Utah

Team Members

Nancy Meyer
Institution
Oregon Health & Science University

Heidi Schubert
Institution
University of Utah