Determination of the structure of an archaeal clamp loading complex
via single particle cryogenic electron microscopy
EMSL Project ID
51769
Abstract
Replicative DNA polymerases associate with ring-shaped sliding clamps that encompass the DNA and therefore allow processive DNA synthesis. Loading of the clamp requires a clamp loading complex that binds and catalyzes the opening and closing of the clamp around the DNA in an ATP-dependent manner. To investigate the mechanism of archaeal clamp loading and the role of ATP hydrolysis in this process, replication factor C (RFC) and its clamp partner, proliferating cell nuclear antigen (PCNA) from S. solfataricus were expressed and co-purified. We have attempted and obtained a preliminary cryogenic electron microscopy (Cryo-EM) structure (8 Å) of the RFC/PCNA complex.
Project Details
Start Date
2020-12-15
End Date
2021-03-17
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members