Cryo-EM Studies of the Cardiac Sodium Channel and Antiarrhythmic Drugs
EMSL Project ID
51781
Abstract
We plan to use cryo-EM to study the cardiac sodium channel Nav1.5 and describe its antiarrhythmic drug receptor in molecular detail. The proposed cryo-EM studies will make use of PNNL’s cutting-edge Titan Krios microscopes to perform single-particle imaging of Nav1.5 in complex with small molecules. Our previously published structures provide support for this approach though PNNL access will accelerate our studies relative to local capabilities and provide the increased resolution needed for these studies. We expect our experiments will increase understanding of cardiac physiology as well as the mechanisms of action for clinically relevant Class IA, IB, and IC antiarrhythmic drugs.
Project Details
Start Date
2020-12-15
End Date
2021-03-17
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members