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Single-particle cryo-EM structural studies of TRPM channels


EMSL Project ID
51815

Abstract

The transient receptor potential melastatin (TRPM) family of ion channels are the largest and most diverse group within the TRP channel superfamily. They are broadly distributed across various tissue and cell types and is involved in regulating diverse physiological processes. Members that serve as key players somatosensation have emerged as promising targets for developing novel therapeutics for treatment of pain and inflammation. To accomplish successful analgesic drug designs, besides functional studies, structural analyses are indispensable for understanding the underlying mechanisms of activation and regulation of these channels. Here we propose to investigate the molecular basis of nociceptive TRPM channels employing protein biochemistry and single-particle cryo-electron microscope as our primary strategies. Our studies will prompt the sensory biology field to progress towards a clearer mechanistic understanding of TRP channel functions.

Project Details

Start Date
2021-02-15
End Date
2021-03-17
Status
Closed

Team

Principal Investigator

Seok-Yong Lee
Institution
Duke University

Team Members

Dohoon Kwon
Institution
Duke University

Ying Yin
Institution
Duke University