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RhizoMap: metabolic imaging of the rhizosphere through time and space


EMSL Project ID
60889

Abstract

Current EMSL technologies for molecular imaging of rhizosphere rely on either analyzing root that has been removed from the soil or on probing microfluidic devices that mimic rhizosphere environment (in a micro-environment setup). Therefore, there is a critical need to develop capabilities for in-situ metabolic mapping of the root-soil-microbe interface under a heterogeneous soil-based conditions. The main objective of the proposal is to develop a MALDI MS imaging approach that will enable temporal tracking and high resolution spatial and molecular information on the metabolite distribution in the rhizosphere. This will be achieved by developing imprinting method and MALDI matrices for imaging vast set of plant and microbial metabolites directly from a custom-built rhizobox filled with soil.

Project Details

Start Date
2023-10-01
End Date
N/A
Status
Active

Team

Principal Investigator

Dusan Velickovic
Institution
Environmental Molecular Sciences Laboratory

Team Members

Kiall Francis Suazo
Institution
Pacific Northwest National Laboratory

Kevin Zemaitis
Institution
Environmental Molecular Sciences Laboratory

Vimal Kumar Balasubramanian
Institution
Environmental Molecular Sciences Laboratory