Kevin Zemaitis

Unlocking the molecular basis of plant-pathogen interactions to create resilient bioenergy crops

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

In the winning 2023-DOE BRAVE (Biopreparedness Research Virtual Environment) Proposal (LAB-23-2955), awarded to co-PI Ljiljana Pasa-Tolic (EMSL/PNNL), PI Qun Liu (BNL), and other co-PI:, Clint Magill…

Spatially resolved characterization of proteoforms for functional proteomics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

Differentiated cells have distinctive patterns of epigenetic marks including various post-translational modifications (PTMs) on histones that may work in concert to control transcriptional programs…

Increased confidence, coverage, and characterization of organic matter through online liquid chromatography hybrid FT-Orbitrap-21T ICR mass spectrometry

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

William Kew

Direct infusion (DI) mass spectrometry analysis of organic matter (OM) is one of EMSL’s primary workflows. It is a powerful and popular means to characterize complex environmental samples. However,…

RhizoMap: metabolic imaging of the rhizosphere through time and space

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Dusan Velickovic

Current EMSL technologies for molecular imaging of rhizosphere rely on either analyzing root that has been removed from the soil or on probing microfluidic devices that mimic rhizosphere environment …

Developing automatic gain control for organic matter analyses by FTICR-MS

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Kevin Zemaitis

Three commercial FTICR-MS instruments (Bruker; 7T, 12T, and 15T) routinely support organic matter (OM) analyses in EMSL. Generally speaking, the quality of data produced is inherently linked to the…

Preserving plant tissue section integrity and introducing ionic organic matrices to strengthen plant MALDI MSI capability at EMSL

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Dusan Velickovic

Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is the most utilized capability for spatial metabolomics in EMSL. Still, it can be significantly improved to…

Development of a nano-desorption atmospheric pressure photoionization (nano-DAPPI) advanced mass spectrometry platform

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Swarup China

Natural organic matter from atmospheric aerosol and soil consist of complex molecules with range of polar and non-polar compounds. Previously EMSL developed a unique platform, nano-desorption…

Advanced 21T FTICR MS instrumentation and methods for functional proteomics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

William Kew

The 21T FTICR-MS at the EMSL provides extremely high mass accuracy and resolution enabling superior depth of characterization for natural organic matter and intact mass measurement of denatured…

Toward plant spatial hormonomics: multiple phytohormone profiling by derivatization assisted-ionization enhanced targeted MALDI MS imaging

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Dusan Velickovic

Phytohormones are physiologically important small molecules that regulate diverse processes as chemical messengers within plants and in plant communications with environments. However, the complex…

The secret life of plants: intact protein mass spectrometry imaging by nanospray desorption electrospray ionization (nanoDESI)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Kevin Zemaitis

Intact protein mass spectrometry imaging (MSI) is a niche field of study which combines the specificity of mass spectrometry (MS) with the capabilities of visualization as in microscopy. Proteomics…

Online, real-time instrument parameter optimization for FT-ICR mass spectrometry

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

William Kew

Optimal and efficient use of FT-ICR mass spectrometers is a critical step to high-throughput and automated analyses, as well as for ensuring the highest fidelity of data and scientific rigor. This…

Development and evaluation of low-temperature plasma (LTP) for organic matter and aerosol analyses by FTICR mass spectrometry

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Kevin Zemaitis

The analyses of various forms of organic matter (OM) and secondary organic aerosols (SOA) by high-resolution mass spectrometry (e.g., FTICR MS) has found great utility in broadly characterizing…

Projects