Projects | Environmental Molecular Sciences Laboratory

Specialized Ribosomes: A New Frontier in Gene Regulation

Lead Institution

Texas A&M University

Principal Investigator

Deborah Bell-Pedersen

Project type

FICUS Research

A longstanding view in molecular biology is that all the ribosomes in a cell function identically. Our recent preliminary data, and those of others, challenge this dogma, and instead support the…

A Rhizosphere-Scale Investigation of the Relationship Between Plant Productivity and Methane Emissions from Wetlands

Lead Institution

University of Washington

Principal Investigator

Rebecca Neumann

Project type

FICUS Research

The proposed project will advance understanding of the response of methane production and methane oxidation to changes in plant productivity so that modeled representations of these processes and…

Integrated Omics Analyses of a Populus Pedigree for Crop Improvement

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

Chongle Pan

Project type

FICUS Research

Populus is a DOE flagship plant for biofuel biomass production. Improving Populus as a feedstock crop is a major focus of DOE Bioenergy Research Centers (BRCs) and many academic laboratories. Here,…

Genomes to dynamic decay communities: Understanding fungal interactions during early decomposition events in natural lignocellulosic substrate

Lead Institution

Swansea University

Principal Investigator

Dan Eastwood

Project type

FICUS Research

Understanding the microbial decomposition of lignocellulose and its contribution to global carbon cycling requires realistic model systems that go beyond in silico and single culture analyses. Fungal…

3D Reality Check: Developing Structural Support for Predicting Microbial Function and Interpreting Microbial "Omics" Data

Lead Institution

Marine Biological Laboratory

Principal Investigator

Zoe Cardon

Project type

FICUS Research

Microbial activities on which humanity and ecosystems depend, and which influence climate and support biofuel production, are determined both by microbial community membership and by the resources…

Investigating the carbon cycling implications of changing microbial leaf litter decomposition across a permafrost thaw gradient.

Lead Institution

University of Arizona

Principal Investigator

Scott Saleska

Project type

FICUS Research

Thawing arctic permafrost (which contains 30-50% of global soil carbon) is expected to drive substantial alterations to carbon (C) cycling that will accelerate climate change. As permafrost thaws,…

Detecting Seismically-Sustained Deep Subsurface CH4-cycling Chemolithoautotrophic Microbial Communities Using Multi-Omic Analyses and NanoSIMS

Lead Institution

Princeton University

Principal Investigator

Tullis Onstott

Project type

FICUS Research

The specific objective of the proposed JGI/EMSL project is to document the microbial ecosystem of an active fault zone at 3.5 to 3.6 kilometers depth in South Africa. More specifically, the goal is…

Characterizing the ecophysiologies of uncultured syntrophic associations driving anaerobic methane oxidation: applications of targeted meta'omics and multi-modal analytical imaging of diverse archaeal-bacterial consortia

Lead Institution

California Institute of Technology

Principal Investigator

Victoria Orphan

Project type

FICUS Research

The main objective of this work is to combine the molecular, elemental and cryo-electron imaging and metaproteomics available at EMSL with the single cell genomics pipeline at the JGI in a highly…

Systems Analysis of Trophic Transitions in Chromochloris zofingiensis, an Emerging Model Green Alga with Promising Biofuel Potential

Lead Institution

University of California, Berkeley

Principal Investigator

Krishna Niyogi

Project type

FICUS Research

As a core component of a sustainable bio-economy, microalgae have the potential to become a major source of biofuels and bioproducts without exacerbating environmental problems. These photosynthetic…

Biodiversity and ice-nucleating activity of soil and marine airborne microorganisms and relationship to source material

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Susannah Burrows

Project type

FICUS Research

Understanding the nature, sources, and geographic and seasonal distribution of atmospheric ice nucleating particles (INPs), i.e., particles that promote freezing of cloud droplets, is critical to…

Towards a molecular-level understanding of terrestrial organic matter transformations by microbes in a rapidly changing Arctic Ocean

Lead Institution

Concordia University

Principal Investigator

David Walsh

Project type

FICUS Research

The fate of terrestrial organic matter in coastal seas and open oceans is not well understood. This is especially true in the Arctic Ocean, which is one of the largest depositories of terrestrial…

Revealing the Molecular Interactions in Phototroph-Heterotroph Co-Cultures for Bioenergy Through Development of an In Silico Platform for Design and Discovery

Lead Institution

Johns Hopkins University

Principal Investigator

Michael Betenbaugh

Project type

FICUS Research

Lichens are nature's solar cells, harnessing energy from sunlight and converting it along with CO2 into biochemicals. They achieve this goal through a complex web of interactions between a…

From phenotype to genotype and back again: large scale functional characterization of microbial dark matter by combining activity-based cell sorting, isotope labeling, and genomic sequencing

Lead Institution

Montana State University

Principal Investigator

Roland Hatzenpichler

Project type

FICUS Research

Two long-standing problems in microbial ecology are: (1) identifying the factors driving metabolic activity of microbes in their natural environment and (2) determining the substrates that are…

Identifying and applying design rules to engineer polyketide synthases for production of biofuels and bioproducts

Lead Institution

Lawrence Berkeley National Laboratory

Principal Investigator

Jay Keasling

Project type

FICUS Research

Polyketide synthases (PKSs) produce numerous secondary metabolites in soil dwelling bacteria. For over two decades, researchers have known that portions of these enzymes can be recombined to produce…

Tracking switchgrass photosynthate via 13CO2 pulse-chase into the rhizosphere microbiome and metabolome

Lead Institution

Michigan State University

Principal Investigator

Lisa Tiemann

Project type

FICUS Research

This project aims to increase our understanding of plant-microbe interactions, specifically the exchange of plant C for microbially derived nitrogen. Plant exudates are a high energy foods for…

Combining high resolution organic matter characterization and microbial meta-omics to assess the effects of nutrient loading on salt marsh carbon sequestration

Lead Institution

Northeastern University

Principal Investigator

Jennifer Bowen

Project type

FICUS Research

Salt marshes sequester an order of magnitude more carbon in their sediments than their terrestrial counterparts because rates of decomposition are inhibited by anoxic, water-logged soils. Once easily…

Scaling molecular mechanisms of mycorrhizal-decomposer interactions to emergent ecosystem carbon balance

Lead Institution

Boston University

Principal Investigator

Jennifer Bhatnagar

Project type

FICUS Research

Emerging evidence from field experiments and global scale analyses suggests that ectomycorrhizal fungi may slow soil carbon (C) cycling via negative interactions with free-living microbial…

The synchronization of microbial and plant phenology in a mountainous watershed and its importance for nutrient retention under changing hydrologic regimes.

Lead Institution

Lawrence Berkeley National Laboratory

Principal Investigator

Eoin Brodie

Project type

FICUS Research

In mountainous systems that experience seasonal snow-cover, the snowmelt period is characterized by a large pulse of nutrients that originates from microbial mineralization of organic matter beneath…

Linking Proteogenomics, Metabolomics, and Soil Organic Chemistry of Tropical Wetlands to a Soil Nutrient Cycling Model

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

Melanie Mayes

Project type

FICUS Research

Our goal is to explicitly link microbial function for decomposition of soil organic matter to substrate organic chemistry to improve process representation in a soil organic carbon and phosphorus (P)…

Engineering enzyme networks for biomass deconstruction in synthetic microbial communities

Lead Institution

University of British Columbia

Principal Investigator

Steven Hallam

Project type

FICUS Research

Lignocellulose, the main component of plant cell walls, is arguably the most abundant biopolymer on the planet1,2. Through biorefining, it is also a renewable input for energy and materials…