Projects

Modeling Multiscale-Multiphase-Multicomponent Subsurface Reactive Flows using Advanced Computing

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Glenn Hammond

Project type

Large-Scale EMSL Research

During the course of the project we will apply PFLOTRAN to uranium migration at the Hanford 300 Area and to CO2 sequestration in subsurface geologic formations. In addition, we will test new…

Conformational Dynamics of Pin1 Regulation of APP processing and Abeta Production

Lead Institution

Cornell University

Principal Investigator

Linda Nicholson

Project type

Large-Scale EMSL Research

The proposed studies fall within the EMSL science theme of "Biological Interactions and Dynamics", specifically in the effort to understand how the "readout", or experimentally…

Mechanistic Studies of Protein Allostery at the Atomistic Scale

Lead Institution

University of California, Berkeley

Principal Investigator

Jhih-Wei Chu

Project type

Large-Scale EMSL Research

Allostery refers to the change of protein conformation in response to ligand binding, biochemical modifications, or interactions with other proteins. In this proposal, we plan to study protein…

STRUCTURAL STUDIES OF REGULATORS OF HISTONE PROTEIN SYNTHESIS

Lead Institution

University of North Carolina

Principal Investigator

Roopa Thapar

Project type

Large-Scale EMSL Research

STRUCTURAL STUDIES OF REGULATORS OF HISTONE PROTEIN SYNTHESIS. Roopa Thapar and William F. Marzluff In each cell cycle a eukaryotic cell must not only replicate its DNA, but must also…

The Aerosol Modeling Testbed

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jerome Fast

Project type

Large-Scale EMSL Research

An Aerosol Modeling Testbed will be developed that will simplify and greatly increase the efficiency of evaluating new aerosol treatments for regional and global climate models. The Aerosol Modeling…

Coupled Hydrological and Geochemical Studies on Uranium Plumes at Hanford Site: Reactions, Spatial Distributions, Speciation, and Future Mobility

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Zheming Wang

Project type

Large-Scale EMSL Research

Massive quantities (exceeding 85 tons) of U currently reside in the Hanford vadose zone, and will threaten groundwater and the accessible environment for generations to come. Present insufficient…

Interfacial Phenomena in Cu Plating

Lead Institution

eMAT Technology LLC

Principal Investigator

Valery Dubin

Project type

Large-Scale EMSL Research

Electroplated Cu on a physical vapor deposited (PVD) Cu seed layer with a PVD Ta-based barrier is now an integrated process for the production of advanced interconnects for integrated circuits (IC),…

Mechanisms of Ubiquitin Transfer and Regulation

Lead Institution

University of Washington

Principal Investigator

Rachel Klevit

Project type

Large-Scale EMSL Research

Protein ubiquitination is a powerful regulatory process that influences nearly every aspect of eukaryotic cell biology. Pathways ranging from cell-cycle progression and differentiation to DNA repair…

Exploring the Proteome of E. coli Following Adaptation to Environmental and Genetic Perturbation

Lead Institution

University of California, San Diego

Principal Investigator

Bernhard Palsson

Project type

Large-Scale EMSL Research

Microorganisms rapidly adapt to changes in the environment which ensures their survival under suboptimal conditions. It is well known that microorganisms display genomic plasticity that enables…

Experimental and Theoretical Investigations on the
Chemistry and Dynamics of Ammonia Borane Dehydrogenation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Autrey

Project type

Large-Scale EMSL Research

Increasing demands for clean energy sources that do not add CO2 and other pollutants to the environment have resulted in increased worldwide interest in the possibilities of a "hydrogen economy…

The proteome of prostate cancer-derived exosomes: Exosomes as biomarker treasure chests

Lead Institution

Erasmus University Medical Center, Rotterdam

Principal Investigator

Guido Jenster

Project type

Large-Scale EMSL Research

Prostate cancer (PCa) is the most common cancer and the second leading cause of cancer death in men. Although considerable progress in the clinical setting has been made in the past decades, early…

Solid-State NMR Structural Studies of HIV-1 CA Protein Assembly

Lead Institution

University of Delaware

Principal Investigator

Tatyana Polenova

Project type

Large-Scale EMSL Research

This is an application in response to the proposal call "Science Theme: Biological Interactions and Dynamics". We request standard, general non-proprietary access to the 900 MHz solid-state…

Mechanisms of nanocrystalline uraninite oxidation by iron minerals under of sulfate-reducing bacteria

Lead Institution

South Dakota School of Mines and Technology

Principal Investigator

Rajesh Sani

Project type

Large-Scale EMSL Research

Uranium (U) is the most common radionuclide in soils, sediments, and groundwater at many sites including U.S. DOE sites and is therefore of particular environmental concern. Subsurface bacteria such…

Comprehensive Analysis Of Post-Translational Modifications (PTMs) In Proteins Involved In Histone Pre-mRNA Processing By High Performance nanoLC-MS

Lead Institution

University of North Carolina

Principal Investigator

Roopa Thapar

Project type

Large-Scale EMSL Research

All eukaryotic mRNAs undergo a series of posttranscriptional processing events that lead to the generation of a mature mRNA transcript which is then translated. These pre-mRNA processing steps…

Structural basis of the lysis clock in bacteriophages: the holin-antiholin interaction of phage lambda

Lead Institution

Texas A&M University

Principal Investigator

Christian Hilty

Project type

Large-Scale EMSL Research

Bacteria are the most abundant organisms in the biosphere, and, after cell division, the second most common fate of bacterial cells is lysis by bacteriophage. For most bacteriophages, the duration…

Proteomic Analysis of Extracellular Appendages Displayed in Desulfovibrio vulgaris

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

Dwayne Elias

Project type

Large-Scale EMSL Research

Sulfate-reducing bacteria makeup a substantial portion of subsurface microbial communities at DOE contaminated sites in particular. They are capable of reducing and immobilizing, soluble U(VI) by…

Phosphoproteomic profiling of breast cancer cell lines

Lead Institution

Erasmus University Medical Center, Rotterdam

Principal Investigator

Arzu Umar

Project type

Large-Scale EMSL Research

Breast cancer is a very heterogeneous disease, consisting of different molecular subtypes. Effective, targeted treatment is available for estrogen receptor (ER) and human epidermal growth factor…

Abiotic reduction of U(VI) by sorbed Fe(II) and the implications for field-scale bioremediation at the Rifle IFC project site

Lead Institution

US Geological Survey, Menlo Park

Principal Investigator

Kate Campbell

Project type

Large-Scale EMSL Research

Bioremediation of uranium-contaminated sites has recently been explored as a potentially effective groundwater treatment option. The injection of an organic carbon substrate such as acetate…

Simultaneous Optical & Chemical Measurements of Aerosols as a Function of Relative Humidity: Bridging Laboratory and Field Measurements to Models

Lead Institution

Los Alamos National Laboratory

Principal Investigator

Manvendra Dubey

Project type

Large-Scale EMSL Research

Interactions of anthropogenic aerosols with solar radiation and clouds and are the largest source of uncertainty in climate change science. The key science gap is a lack of process-level knowledge…

The Influence of different Fe(III) Phases and the Effect of Sulfate Reduction on the Simultaneous Bioreduction of Iron and Uranium

Lead Institution

Princeton University

Principal Investigator

Peter Jaffe

Project type

Large-Scale EMSL Research

Biostimulation of Fe(III) and/or sulfate reducing microorganisms by addition of acetate can effectively reduce U(VI). However, U(VI) removal from groundwater was hindered in field trials at the…