Barry Stoddard

Collaborative Access Team: Pacific Northwest Cryo-EM Center (PNCC)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

PNNL will create and operate the Pacific Northwest Center for Cryo-EM (PNCC), a state-of-the-art national center for cryo-electron microscopy (Cryo-EM) that will rapidly and efficiently collect and…

Structural characterization of restriction/modification enzyme BsaXI complex with DNA target for structure-based design of gene-targeting reagents

Lead Institution

Fred Hutchinson Cancer Research Center

Principal Investigator

Betty Shen

Genome engineering and targeted gene modification is a rapidly maturing discipline in which genomes within cell lines, tissues or organisms are manipulated and altered at specified individual loci…

CryoEM structural determinations of Bacterial Restriction-Modification systems

Lead Institution

Fred Hutchinson Cancer Research Center

Principal Investigator

Betty Shen

Nuclease enzymes are of enormous importance for a wide range of biological and biotechnology disciplines that require site-specific DNA manipulations. Bacterial R-M systems consist of a restriction…

Cryo-EM characterization of functional components of a Bacteriophage exclusion system

Lead Institution

Fred Hutchinson Cancer Research Center

Principal Investigator

Betty Shen

The strategies employed by bacteria and archaea for combatting phage infection, and the corresponding ‘arms race’ between phage and their microbial targets, have generated a diverse arsenal of innate…

Structural characterization of phage defense protein BrxL

Lead Institution

Fred Hutchinson Cancer Research Center

Principal Investigator

Barry Stoddard

Bacterial defense against phage infection is comprised of a large number of biological mechanisms and systems. A thorough understanding of such systems is crucial both for anti-bacterial phage…

EMSL Science Overview

Barry Stoddard

Projects

Collaborative Access Team: Pacific Northwest Cryo-EM Center (PNCC)

Structural characterization of restriction/modification enzyme BsaXI complex with DNA target for structure-based design of gene-targeting reagents

CryoEM structural determinations of Bacterial Restriction-Modification systems

Cryo-EM characterization of functional components of a Bacteriophage exclusion system

Structural characterization of phage defense protein BrxL