Isaac Attah

Development of an Environmental-Focused DIA Pipeline for HTP Proteomics

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Isaac Attah

Currently, metaproteomics workflows are hindered by sample complexity which necessitates fractionation of the sample meaning one sample can require 12 to 24 MS analyses. Data dependent workflows …

Identification of HLA-A29 peptides bound to the MHC-Class I groove in birdshot uveitis patient leukocytes.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Melchior

Birdshot chorioretinopathy (BSCR) is an autoimmune disorder that causes blindness. All patients with BSCR have the HLA-A29 allele encoded by the HLA-A29 MHC Class I receptor. However, less than 1%…

Pentosan Polysulfate Sodium Profiling with Mass Spectrometry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Cort

PPS is a semisynthetic heterogeneous sulfated polysaccharide derived from xylan, the β-1,4-linked polymer of xylose that is the backbone structural component of hemicellulose in most types of wood…

Predicting adverse biological effects for chemicals of concern through integration of functional profiling with artificial intelligence/machine learning (AI/ML)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vivian Lin

We will use thermal proteome profiling (TPP) to identify protein targets and pathways affected by exposure to a chemical of concern (COC). As a next step, we will use an artificial intelligence…

Pentosan Polysulfate Sodium Profiling with Mass Spectrometry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Cort

PPS is a semisynthetic heterogeneous sulfated polysaccharide derived from xylan, the β-1,4-linked polymer of xylose that is the backbone structural component of hemicellulose in most types of wood…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Proteomics analysis of ovulation in primates

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Sneha Couvillion

To perform a parallel genomic (RNAseq, University of Oregon) and proteomic (PNNL) assessment of the ovarian follicle in the period prior to and at different time points after an ovulatory stimulus (i…

Structural profiling of the molecular interactome to define phenotype

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Melchior

The functional pathways that define phenotype are reflected by protein quinary structure; the transient encounters of proteins with other proteins, molecules, cofactors, and nucleic acids that…

EMSL Science Overview

Isaac Attah

Projects

Development of an Environmental-Focused DIA Pipeline for HTP Proteomics

Identification of HLA-A29 peptides bound to the MHC-Class I groove in birdshot uveitis patient leukocytes.

Pentosan Polysulfate Sodium Profiling with Mass Spectrometry

Predicting adverse biological effects for chemicals of concern through integration of functional profiling with artificial intelligence/machine learning (AI/ML)

Pentosan Polysulfate Sodium Profiling with Mass Spectrometry

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Proteomics analysis of ovulation in primates

Structural profiling of the molecular interactome to define phenotype