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M. Mayer-Cumblidge

Institution
Pacific Northwest National Laboratory

Projects

Genomes-to-Life Protein Complex Isolation Capability

Lead Institution
Western University of Health Sciences
Principal Investigator
Thomas Squier
Project type
Exploratory Research
We have established a pilot proteomics facility in support of the "Goal 1" of the Department of Energy's Genomes to Life program, which seeks to identify 80% of the protein complexes…

Development of Cell Permeable Dyes for in-vivo Live Cell Imaging

Lead Institution
Western University of Health Sciences
Principal Investigator
Thomas Squier
Project type
Exploratory Research
. Our long-term goal is to develop the necessary reagents and technology for the rapid identification of a substantial fraction of the multiprotein complexes in an organism, thereby permitting a…

Molecular Modeling

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
M. Mayer-Cumblidge
Project type
Exploratory Research
We are working on the design and synthesis of Arsenic-derivatized dyes and crosslinkers which selectively bind multi-cysteine peptide motifs. These motifs can then be cloned into proteins of…

Protein Complex Identification using novel affinity resins

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
M. Mayer-Cumblidge
Project type
Exploratory Research
We are validating the use of a FlAsH resin with a dithiol releasing agent for high-throughput protein complex identification We are going to do endogenous pull-downs of protein complexes from S…

Protein Complex Identification using novel affinity resins

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
M. Mayer-Cumblidge
We are validating the use of a FlAsH resin with a dithiol releasing agent for high-throughput protein complex identification We are going to do endogenous pull-downs of protein complexes from S…

NMR of small organics

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
M. Mayer-Cumblidge
Project type
Capability Research
We are synthesizing small organic crosslinkers and dye derivatives. We would like to analyze their composition and purity by 1D proton (and potentially carbon) NMR in deuterated solvents. In most…

Dye Studies on CHO cells.

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
M. Mayer-Cumblidge
Project type
Exploratory Research
We have synthesized two dyes (FlAsH and ReAsH)which bind to a tetracysteine motif. We are seeking to optimize binding to a protein in living cells.