Protein Complex Identification using novel affinity resins
EMSL Project ID
12091a
Abstract
We are validating the use of a FlAsH resin with a dithiol releasing agent for high-throughput protein complex identification We are going to do endogenous pull-downs of protein complexes from S. oneidensis and validations in E. coli and would like to use LCMS-MS/SEQUEST to identify the proteins in our complex mixtures. We will be submitting trypsinized peptides from protein mixtures, in which 2-20 proteins should be highly enriched. We expect that in terms of mass spectrometry these samples will be well-suited to the classic Shewanella federation run times and approaches. In total, we estimate between 60 and 150 samples in this fiscal year.
Project Details
Start Date
2006-11-07
End Date
2009-11-08
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Yan P, T Wang, GJ Newton, TV Knyushko, Y Xiong, DJ Bigelow, TC Squier, and MU Mayer. 2009. "A Targeted Releasable Affinity Probe (TRAP) for In Vivo Photo-Crosslinking." Chembiochem 10(9):1507-1518. doi:10.1002/cbic.200900029