Skip to main content

Thomas Squier

Institution
Western University of Health Sciences

Projects

Role of Confinement and Material Surface on Protein Dynamics and Function

Lead Institution
Washington State University Tri-Cities
Principal Investigator
Kathleen McAteer
Project type
Limited Scope
Our goal is to develop a mechanistic understanding of optimal material properties that would permit the functional stabilization of broad classes of proteins with utility to a range of detection and…

Role of Confinement and Material Surface on Protein Dynamics and Function

Lead Institution
Washington State University Tri-Cities
Principal Investigator
Kathleen McAteer
Project type
Limited Scope
Our goal is to develop a mechanistic understanding of optimal material properties that would permit the functional stabilization of broad classes of proteins with utility to a range of detection and…

Dynamic Imaging of Structurally Heterogeneous Protein Complexes

Lead Institution
Western University of Health Sciences
Principal Investigator
Thomas Squier
Project type
Large-Scale EMSL Research
Our long-term goal is to identify biological design principles that will enable synthetic biology approaches for bioenergy applications. We hypothesize that the targeted engineering of selected…

Protein Complex Identification using novel affinity resins

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
M. Mayer-Cumblidge
Project type
Exploratory Research
We are validating the use of a FlAsH resin with a dithiol releasing agent for high-throughput protein complex identification We are going to do endogenous pull-downs of protein complexes from S…

Protein Complex Identification using novel affinity resins

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
M. Mayer-Cumblidge
We are validating the use of a FlAsH resin with a dithiol releasing agent for high-throughput protein complex identification We are going to do endogenous pull-downs of protein complexes from S…

Genomes-to-Life Protein Complex Isolation Capability

Lead Institution
Western University of Health Sciences
Principal Investigator
Thomas Squier
Project type
Exploratory Research
We have established a pilot proteomics facility in support of the "Goal 1" of the Department of Energy's Genomes to Life program, which seeks to identify 80% of the protein complexes…

The Evolution of Protein Flexibility

Lead Institution
University of Idaho
Principal Investigator
Gary Daughdrill
Project type
Capability Research
When novel genes are sequenced their structure and function can often be reliably predicted based on sequence similarity and evolutionary relationships to proteins with known structures. Currently,…

Collaborative Research Projects Supported by the NCRR P41 Resource Center

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
David Camp
Project type
Large-Scale EMSL Research
The Proteomics Resource Center for Integrative Biology at PNNL was established in 2003 and builds on the unique instrumentation and the infrastructure provided by PNNLs EMSL facility. The mission of…

NMR of small organics

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
M. Mayer-Cumblidge
Project type
Capability Research
We are synthesizing small organic crosslinkers and dye derivatives. We would like to analyze their composition and purity by 1D proton (and potentially carbon) NMR in deuterated solvents. In most…

Regulatory dynamics of microbial communities

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
H Wiley
Project type
Large-Scale EMSL Research
This is a proposal to conduct work in EMSL in support of the Foundational Scientific Focus Area (FSFA) research program at Pacific Northwest National Laboratory. This large-scale research program…

Development of Cell Permeable Dyes for in-vivo Live Cell Imaging

Lead Institution
Western University of Health Sciences
Principal Investigator
Thomas Squier
Project type
Exploratory Research
. Our long-term goal is to develop the necessary reagents and technology for the rapid identification of a substantial fraction of the multiprotein complexes in an organism, thereby permitting a…

Electron and Proton Transfer Reactions in Photobiological H2 Production

Lead Institution
Universidade Federal de Pernambuco
Principal Investigator
Thereza Soares Da Silva
Project type
Capability Research
The biological photoproduction of hydrogen by microbial photosynthetic organism requires light as the energy source, an electron-donating substrate, and a catalyst that combines electrons and protons…