Timothy Donohue

High-resolution imaging of Rhodobacter sphaeroides strains with increased lipid accumulation

Lead Institution

University of Wisconsin, Madison

Principal Investigator

Timothy Donohue

Project type

Special Science

We are requesting access to quiet wing microscopy, including ETEM and STEM electron microscopes and the helium ion microscope, as well as the super resolution structured illumination fluorescence and…

Biosystem dynamics and analysis of Rhodobacter sphaeroides strains with increased lipid accumulation

Lead Institution

University of Wisconsin, Madison

Principal Investigator

Timothy Donohue

Project type

Large-Scale EMSL Research

We are requesting access to EMSL microscopy, including ETEM and STEM electron microscopes and the helium ion microscope, as well as the super resolution structured illumination fluorescence and multi…

Biosystem dynamics and analysis of Rhodobacter sphaeroides strains with increased lipid accumulation

Lead Institution

University of Wisconsin, Madison

Principal Investigator

Timothy Donohue

Project type

Large-Scale EMSL Research

We are requesting access to EMSL microscopy, including transmission electron microscopes, the helium ion microscope, and atomic force microscope. We also are requesting access to high-resolution…

High-resolution imaging of Rhodobacter sphaeroides strains with increased lipid production

Lead Institution

University of Wisconsin, Madison

Principal Investigator

Timothy Donohue

Project type

Large-Scale EMSL Research

Rhodobacter sphaeroides is an alpha-proteobacterium which can remodel its cell envelope under low-oxygen or anaerobic conditions, by increasing its lipid content and forming intracytoplasmic membrane…

Application of High-Throughput Proteomics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mary Lipton

The understanding of how cells function at the systems level will greatly benefit from the development of new approaches capable of making global measurements of protein expression (proteome). The…

Analysis of zinc environments in singlet oxygen-responsive proteins

Lead Institution

University of Wisconsin, Madison

Principal Investigator

Timothy Donohue

Project type

Capability Research

This project will use solid state NMR to probe the binding and environment of zinc ligands in the ChrR protein from the photosynthetic bacterium Rhodobacter sphaeroides. ChrR is a zincmetalloprotein…

Rhodobacter sphaerodies

Lead Institution

University of Wisconsin, Madison

Principal Investigator

Timothy Donohue

Project type

Exploratory Research

Rhodobacter sphaerodies 2.4.1. is a-3 purple nonsulfur eubacterium with an extensive metabolic repertoire. Under anaerobic conditions, it is able to grow by photosynthesis, respiration and…

High-resolution imaging of Rhodobacter sphaeroides strains with increased lipid accumulation

Lead Institution

University of Wisconsin, Madison

Principal Investigator

Timothy Donohue

Project type

Exploratory Research

We are requesting access to super resolution fluorescence and electron microscopy/imaging facilities at EMSL. We propose to utilize these facilities to obtain molecular and high-resolution insights…

EMSL Science Overview

Timothy Donohue

Projects

High-resolution imaging of Rhodobacter sphaeroides strains with increased lipid accumulation

Biosystem dynamics and analysis of Rhodobacter sphaeroides strains with increased lipid accumulation

Biosystem dynamics and analysis of Rhodobacter sphaeroides strains with increased lipid accumulation

High-resolution imaging of Rhodobacter sphaeroides strains with increased lipid production

Application of High-Throughput Proteomics

Analysis of zinc environments in singlet oxygen-responsive proteins

Rhodobacter sphaerodies

High-resolution imaging of Rhodobacter sphaeroides strains with increased lipid accumulation