Skip to main content

Analysis of zinc environments in singlet oxygen-responsive proteins


EMSL Project ID
21493

Abstract

This project will use solid state NMR to probe the binding and environment of zinc ligands in the ChrR protein from the photosynthetic bacterium Rhodobacter sphaeroides. ChrR is a zincmetalloprotein that acts as the master regulator of a transcriptional response to singlet oxygen, a toxic, reactive oxygen species that is a byproduct of solar energy capture in this and all photosynthetic organisms. By analyzing zinc binding to ChrR and its microenvironment, we will obtain critical new information into the zinc binding by this protein and the role of this metal in a biological response to singlet oxygen that is widely conserved in microbes

Project Details

Project type
Capability Research
Start Date
2006-10-31
End Date
2008-09-18
Status
Closed

Team

Principal Investigator

Timothy Donohue
Institution
University of Wisconsin, Madison

Team Members

Andrew Lipton
Institution
Environmental Molecular Sciences Laboratory