Analysis of zinc environments in singlet oxygen-responsive proteins
EMSL Project ID
21493
Abstract
This project will use solid state NMR to probe the binding and environment of zinc ligands in the ChrR protein from the photosynthetic bacterium Rhodobacter sphaeroides. ChrR is a zincmetalloprotein that acts as the master regulator of a transcriptional response to singlet oxygen, a toxic, reactive oxygen species that is a byproduct of solar energy capture in this and all photosynthetic organisms. By analyzing zinc binding to ChrR and its microenvironment, we will obtain critical new information into the zinc binding by this protein and the role of this metal in a biological response to singlet oxygen that is widely conserved in microbes
Project Details
Project type
Capability Research
Start Date
2006-10-31
End Date
2008-09-18
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members