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Structural Biology of the Human High Mobility Group A (HMGA) Proteins: Characterizing the Hub of Nuclear Function


EMSL Project ID
1620a

Abstract

Use MS to identify sites and types of post-translational modifications of several high mobility grop proteins. Use various tandem mass spec instruments available to precisely identify specific residues that are modified. Proteins were purified from celular extracts using RP-HPLC. Initial characterization of tryptic digests are the proteins using MALDI MS at WSU suggests that there are sites of methylation, acetylation, and phosphorylation on these proteins. Yet pinning down specific sites and types of modifications is difficult without use of a tandem MS. Total of 6 proteins needto be immediately analyzed with 2 more in near future. The tryptic digests of all the proteins are complete and ready for analysis.

Project Details

Project type
Capability Research
Start Date
2004-12-29
End Date
2006-04-04
Status
Closed

Team

Principal Investigator

Raymond Reeves
Institution
Washington State University

Team Members

Garry Buchko
Institution
Pacific Northwest National Laboratory

Michael Kennedy
Institution
Miami University