43Ca NMR Spectroscopy of Ca2+ Dependent Proteins and models
EMSL Project ID
25710
Abstract
We are exploring the application and utility of low temperature solid-state NMR methods to the observation of 43Ca in Ca2+ dependent proteins. Our first application is to the protein calbindin D9k (the E27QP43M mutant). Based on the data obtained we surmise that such experiments utilizing modestly enriched Ca2+ (5 to 20% enrichment levels) should be of comparable difficulty to existing applications involving 25Mg dependent proteins.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2007-06-01
End Date
2009-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Lipton AS, PD Ellis, and TE Polenova. 2009. "Quadrupolar Metal Nuclides in Bioinorganic Chemistry: Solid-State NMR Studies." In Encyclopedia of Magnetic Resonance. John Wiley & Sons, Ltd., Hoboken, NJ.