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43Ca NMR Spectroscopy of Ca2+ Dependent Proteins and models


EMSL Project ID
25710

Abstract

We are exploring the application and utility of low temperature solid-state NMR methods to the observation of 43Ca in Ca2+ dependent proteins. Our first application is to the protein calbindin D9k (the E27QP43M mutant). Based on the data obtained we surmise that such experiments utilizing modestly enriched Ca2+ (5 to 20% enrichment levels) should be of comparable difficulty to existing applications involving 25Mg dependent proteins.

Project Details

Project type
Large-Scale EMSL Research
Start Date
2007-06-01
End Date
2009-09-30
Status
Closed

Team

Principal Investigator

Paul Ellis
Institution
Pacific Northwest National Laboratory

Team Members

Robert Heck
Institution
Pacific Northwest National Laboratory

Andrew Lipton
Institution
Environmental Molecular Sciences Laboratory

John Hanna
Institution
University of Warwick

Related Publications

Lipton AS, PD Ellis, and TE Polenova. 2009. "Quadrupolar Metal Nuclides in Bioinorganic Chemistry: Solid-State NMR Studies." In Encyclopedia of Magnetic Resonance. John Wiley & Sons, Ltd., Hoboken, NJ.