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Proteomics Analysis for the Center for Novel Biomarkers of Response


EMSL Project ID
33706

Abstract

The organizing theme for the PNNL U54 Center for Novel Biomarkers of Response, is that identification and validation of persistent modified proteins in plasma will provide specific information about the stressor, its mode of action, and the target organ. The environmental stress of the human and animal projects is focused on main-stream and side-stream cigarette smoke with obesity as a contributing physiological factor. The additional specificity provided by the biosignatures of modified proteins will eventually allow assessment of susceptibility and better elucidate the relationship between genes, exposure, and human disease. This integrated, multidisciplinary center contains two research projects (human and mouse) with proteomics as a technology core that will support the identification and validation of nitrotyrosine and related post translational modifications of proteins as biomarkers of cigarette-smoke induced inflammatory/oxidative stress.

Project Details

Start Date
2009-12-03
End Date
2012-12-09
Status
Closed

Team

Principal Investigator

Jon Jacobs
Institution
Environmental Molecular Sciences Laboratory

Team Members

Uma Aryal
Institution
Pacific Northwest National Laboratory

Xu Zhang
Institution
Pacific Northwest National Laboratory

Joel Pounds
Institution
Pacific Northwest National Laboratory

Related Publications

Matzke MM, KM Waters, TO Metz, JM Jacobs, A Sims, R Baric, JG Pounds, and BJM Webb-Robertson. 2011. "Improved Quality Control Processing of Peptide-centric LC-MS Proteomics Data." Bioinformatics.
Webb-Robertson BJM, LA McCue, KM Waters, MM Matzke, JM Jacobs, TO Metz, SM Varnum, and JG Pounds. 2010. "Combined Statistical Analyses of Peptide Intensities and Peptide Occurrences Improves Identification of Significant Peptides from MS-based Proteomics Data." Journal of Proteome Research 9(11):5748-5756.