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Establishment of a Robust Method for Soil Metabolomics Analysis


EMSL Project ID
49530

Abstract

The objective of this work is to adapt current evolving protocols for metabolomics analyses to soil samples. As chemical perturbations are primarily detected on a molecular level, the metabolome will reveal the biological response to the physicochemical influence of soil, ultimately leading to a more complete understanding of the factors driving microbial diversity within soil. The goals of this experiment are as follows: 1) Establish conditions for microbial growth within treated soil cores, 2) determine optimal extraction protocols to capture analytes, 3) unravel the metabolic processes such as cell to cell signaling, and cross feeding through GC-MS, and 1H NMR, and 4) extrapolate meaningful biological metabolic networks within soil from data.

Project Details

Project type
Limited Scope
Start Date
2016-07-21
End Date
2016-09-20
Status
Closed

Team

Principal Investigator

Vanessa Bailey
Institution
Pacific Northwest National Laboratory

Co-Investigator(s)

Young-Mo Kim
Institution
Pacific Northwest National Laboratory

Team Members

Thomas Metz
Institution
Pacific Northwest National Laboratory