Establishment of a Robust Method for Soil Metabolomics Analysis
EMSL Project ID
49530
Abstract
The objective of this work is to adapt current evolving protocols for metabolomics analyses to soil samples. As chemical perturbations are primarily detected on a molecular level, the metabolome will reveal the biological response to the physicochemical influence of soil, ultimately leading to a more complete understanding of the factors driving microbial diversity within soil. The goals of this experiment are as follows: 1) Establish conditions for microbial growth within treated soil cores, 2) determine optimal extraction protocols to capture analytes, 3) unravel the metabolic processes such as cell to cell signaling, and cross feeding through GC-MS, and 1H NMR, and 4) extrapolate meaningful biological metabolic networks within soil from data.
Project Details
Project type
Limited Scope
Start Date
2016-07-21
End Date
2016-09-20
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members