High Resolution High Throughput Proteomics Platform for Cancer Research
EMSL Project ID
50126
Abstract
The objective of this project is to develop and evaluate a new high-throughput and ultra-sensitive platform that will enable more effective and higher throughput proteomics measurements to support and advance cancer research. The new platform will provide substantially higher resolution ion mobility spectrometry (IMS) separations than current platforms. The resolution of the IMS achieved will be greatly increased by using electric traveling waves applied to an extremely long path design based upon Structures for Lossless Ion Manipulations (SLIM), while also enabling very high sensitivity to be achieved. The platform will provide approximately two orders of magnitude improvement in measurement throughput for similar or better measurement coverage, quantification, etc. over conventional condensed-phase liquid chromatography-mass spectrometry (LC-MS) platforms, enabling thousands of samples to be analyzed in hours, rather than weeks as at present. We will also adapt and refine bioinformatics tools to provide automated and efficient data processing. The platform will be evaluated utilizing complex samples in terms of proteomic peptide/protein coverage, measurement throughput, CVs of replicate analyses and platform robustness and in comparison to conventional LC-MS platforms.
Project Details
Start Date
2017-12-20
End Date
2018-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members