HIV capsid retrograde trafficking complex
EMSL Project ID
51354
Abstract
After attaching to the cell surface and membrane fusion, HIV releases the viral capsid into the target cells, which is transported to the cell nucleus. Evidence suggests that dynein-dynactin-BicD2 complex plays key roles in this retrograde movement. It is reported that BicD2 can bind to the HIV capsid directly. However, the exact binding region and mechanisms are still unclear. We have assembled the complex of BicD2 with the HIV capsid protein hexamer. Elucidating the structure of this complex would significantly advance the understanding of how HIV capsid is trafficked. We have worked out the cryo grid condition and collected a preliminary cryo-EM data set that demonstrated the suitability for high-resolution reconstruction with a larger data set. However, the Yale Titan Krios recently suffered mechanical failures and is scheduled for the Gatan K3 detector upgrade in March. It is anticipated that the microscope will be offline for the next several months.
Project Details
Start Date
2020-05-15
End Date
2020-11-15
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members