Cryo-EM analysis of the C. perfringens toxin TpeL with its host cell receptor LRP1
EMSL Project ID
51489
Abstract
Clostridioides difficile infection is often activated by alterations in the gut microbiota through antibiotic use, and recurrent infections are associated with severe clinical outcomes. Pathogenesis is mediated by the large clostridial toxins, and intoxication of the host cell proceeds via toxin binding to one or more host cell receptors. The homologous glucosyltransferase exotoxins TpeL from Clostridium perfringens and TcdB from C. difficile share ~30% sequence identity, but TpeL lacks the canonical C-terminal receptor binding domain. At least three receptors have been identified for TcdB, while only one receptor, the LDL receptor-related protein 1, has been shown to bind TpeL, but the details of this interaction are unresolved. We aim to structurally characterize the TpeL:LRP1 complex to determine the molecular interface of this interaction and further our understanding of large clostridial toxin receptor binding specificity.
Project Details
Start Date
2020-09-15
End Date
2021-03-17
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members