Proteomics of Membrane Protein Complexes
EMSL Project ID
5593
Abstract
A comprehensive understanding of the effects of cellular stress on calcium signaling requires correlated measurements of intracellular calcium transients and global proteomic analysis of both calcium-signaling protein complexes and post-translational modifications of proteins. The proposed work aims to develop technical capabilities in proteomic analyses of both 1) membrane protein complexes, and 2) post-translational modifications, areas currently underdeveloped. This work will use two biological systems as experimental models: first, proteins interacting with the voltage-dependent calcium channel from mouse-brain synaptosomes, and second, skeletal muscle cells exposed to oxidative stress to provide an abundant source of oxidatively modified proteins.
Project Details
Project type
Exploratory Research
Start Date
2004-01-21
End Date
2006-01-31
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members