Accelerating cryo-EM screening at EMSL by 25-fold
EMSL Project ID
60907
Abstract
Cryo-Electron Microscopy (cryo-EM) is a powerful structural biology technique since it does not require crystallization (unlike X-ray), does not require ultrahigh purity (unlike NMR) and provides direct few-Å resolution information of all atoms (unlike EPR or APT). It also permits whole cell observation of every density from lipids, DNA/RNA and proteins (unlike super-resolution microscopy which only resolves signal from fluorescently labeled structures). While aspects of the cryo-EM workflow are automated, sample generation and grid freezing optimization remain critical bottlenecks and for many samples are the most time-consuming steps. Here we propose to accelerate the cryo-EM screening and vitrification optimization stages by developing reproducible methods for depositing a 5x5 array on a single cryo-EM grid to increase throughput 25-fold.
Project Details
Start Date
2023-10-01
End Date
N/A
Status
Active
Released Data Link
Team
Principal Investigator
Team Members