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James Evans

Institution
Environmental Molecular Sciences Laboratory

Projects

Accelerating cryo-EM screening at EMSL by 25-fold

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
James Evans
Cryo-Electron Microscopy (cryo-EM) is a powerful structural biology technique since it does not require crystallization (unlike X-ray), does not require ultrahigh purity (unlike NMR) and provides…

Project Title: Protein Structure Without Purification

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
James Evans
Purification remains a major technical and temporal bottleneck for protein annotation especially for membrane, filamentous and heterocomplex assembling proteins. If a target protein in a mixed…

Manganese biomineralization by the bacterial protein complex Mnx

Lead Institution
University of Washington
Principal Investigator
Bradley Tebo
Project type
Large-Scale EMSL Research
Manganese-oxide mineral formation by bacterial oxidation of dissolved Mn(II) is an important process in aquatic and soil environments and is a key part of the global Mn cycle. The Mn bio-oxides…

Dynamic TEM

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
James Evans
Achieving a holistic, molecular-level understanding of the structure, function and mechanism of cellular machinery remains a formidable scientific challenge. While over 100,000 protein structures…

Tomography of Pseudarthrobacter chlorophenolicus

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
James Evans
Subcellular structures and macromolecular complexes can be revealed through cryo electron tomography of whole cells. Pseudarthrobacter chlorophenolicus is a soil bacterium which has novel metabolic…

Towards proteome-wide assessment of cellular landscapes

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Ljiljana Pasa-Tolic
We will develop a novel workflow to characterize proteome-wide structural changes of cellular landscapes between different cell states (or cell types). The goal is to bridge the gap between cellular…

Integrated multi-omic analysis of high lipid accumulation in the oleaginous green microalgae Ostreococcus tauri to engineer metabolic pathways for improved triacylglycerol feedstocks

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
James Evans
Project type
Exploratory Research
Our proposed mutli-omic approach will empower a comprehensive understanding of cellular pathways linking carbon, nitrogen and phosphate metabolism in the microalga Ostreococcus tauri. The use of this…

Visualizing pathways in cells for more accurate biodesign

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
James Evans
In this Capability Development Focus project, we plan to acquire label-free whole cell three-dimensional (3D) tomograms of the two primary organisms of interest for the FY18 Subcellular SSA thrust…

Collaborative Access Team: Pacific Northwest Cryo-EM Center (PNCC)

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
James Evans
PNNL will create and operate the Pacific Northwest Center for Cryo-EM (PNCC), a state-of-the-art national center for cryo-electron microscopy (Cryo-EM) that will rapidly and efficiently collect and…

Joining Systems Biology and Bioimaging on Same Cells

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
James Evans
We plan to develop a new capability at EMSL for coupling bioimaging and systems biology analysis of the same exact cells. The use of the highly simplified and smallest free-living eukaryote…

PNCC Database Capability Development

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
James Evans
This is a capability development proposal to work on the data transfer between PNCC in Oregon and MyEMSL.