Systems Biology Through an Integrated Multimodal Imaging and Analysis Framework
EMSL Project ID
48522
Abstract
Organisms have evolved a number of spatially localized processes to efficiently break down, absorb, and use necessary metabolites. Although progress has been made in identifying the genes that are involved in metabolic processes, determining how compartments are assembled and how proteins are spatially regulated to optimize their function remains mostly obscure. Imaging technologies are ideally suited to investigate spatially regulated processes and can reveal information from the atomic scale to the mesoscale, depending on the particular technology. However, assimilating data from a multitude of separately optimized approaches comprising different data structures to yield generalizable and fundamental knowledge about a particular biophysical/biochemical process is particularly challenging. We propose to meet this challenge by building a multimodal imaging platform that combines a number of different in-situ imaging modalities with unique chemical probes to quantify the dynamics of cellular processes in unprecedented detail. To drive the development of this imaging platform, we will investigate the response of the growing fungal hyphae tip to nutrient changes.
Project Details
Start Date
2014-06-18
End Date
2014-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
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