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Structure and Dynamics of aB57.


EMSL Project ID
7600

Abstract

alphaB-crystallin (aB) is the basic B-subunit of alpha-crystallin, a lenticular protein that is essential for maintaining eye-lens transparency. aB is found associated with a variety of neuro-degenerative diseases such as Alzheimer's disease, Lewy-body disease, etc. Determination of the structure of aB-crystallin by X-ray crystallography has proved elusive for the past three decades due to its polydisperse, oligomeric nature. We propose to tackle the structure of the oligomer with NMR in incremental steps starting from the 100-residue core domain which forms a 23 kDa dimer and ending at the ~650 kDa oligomer. This challenging project requires the best the field of NMR can offer. Hence we are requesting time on the higher field instruments (600 MHz for triple-resonance experiments and relaxation studies on the core domain) at PNNL.

Project Details

Project type
Capability Research
Start Date
2004-05-19
End Date
2006-04-03
Status
Closed

Team

Principal Investigator

Ponni Rajagopal
Institution
University of Washington

Team Members

Rachel Klevit
Institution
University of Washington