Proteomic studies of growth and metabolism in Corynebacterium glutamicum R
EMSL Project ID
9800
Abstract
Corynebacterium glutamicum is a soil bacterium isolated in Japan in the 1950's as an L-glutamic acid producer (Kinoshita, et al., 1957). Strains derived from C. glutamicum are used in a number of industrial amino acid producing processes and recently, an annotated and complete genome sequence was published (Kalinowski et al., 2003). A group at the Research Institute of Innovative Technology for the Earth (RITE) has developed a process termed the "living-cell reaction" in which an isolate of C. glutamicum (termed Cornynebacterium glutamicum R) does not undergo cell division, but remains alive, secreting amino acids, organic acids or ethanol (depending on the strain used) with it's growth and metabolism effectively uncoupled (Terasawa, et al., 1989, 1990, 1991). Using the living-cell reaction process we propose proteomic studies to characterize the interaction cell growth and metabolism pathways in C. glutamicum R. Initially, the proteome of C. glutamicum R maintained under various conditions (including the living-cell reaction conditions [growth suppressed conditions]) will be described and an Accurate Mass Tagged (AMT) database created. The AMT database will serve as a valuable tool for future proteomics studies.
Project Details
Project type
Exploratory Research
Start Date
2004-11-19
End Date
2007-09-02
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
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Understanding the nature of surface nitrates in BaO/γ-Al2O3 NOx storage materials: A combined experimental and theoretical study
Ja Hun Kwak a, Donghai Mei a,∗, Cheol-Woo Yi a,1, Do Heui Kima, Charles H.F. Peden a, Lawrence F. Allard b,
János Szanyi a,∗
a Institute for Interfacial Catalysis, Pacific Northwest National Laboratory, P.O. Box 999, MSIN K8-80, Richland, WA 99352, USA
b High Temperature Materials Laboratory, Oak Ridge National Laboratory, TN, USA
Journal of Catalysis 261 (2009) 17-22.
Verrier CLM, JH Kwak, DH Kim, CHF Peden, and J Szanyi. 2008. "NOx uptake on alkaline earth oxides (BaO, MgO, CaO and SrO) supported on γ-Al2O3." Catalysis Today 136(1-2):121-127. doi:doi:10.1016/j.cattod.2007.12.138