Projects

Developing a Modular Scaffold to Drive the Symmetric Assembly of Cellular Proteins for Single Particle Cryo-EM Imaging

Lead Institution

University of California, Los Angeles

Principal Investigator

Todd Yeates

Project type

Large-Scale EMSL Research

High resolution structural studies can provide exceptionally valuable information on the workings of cellular components and molecular machines, many of which play critical roles in biological energy…

The Transformation of Tetrahedral to Octahedral Coordination in Anions of Interest in High-Level Tank Waste

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jian-zhi Hu

Project type

Large-Scale EMSL Research

We propose to use nuclear magnetic resonance (NMR), Raman and Fourier transform-infrared (FT-IR) spectroscopy in combination with computational modeling to study the conversion of soluble tetrahedral…

System Approaches to Study Soybean Root Biology at Single-Cell Resolution

Lead Institution

University of Missouri - Columbia

Principal Investigator

Gary Stacey

Project type

Large-Scale EMSL Research

The long-term goal of this project is to further basic understanding of the agronomically important Bradyrhizobium japonicum-soybean nitrogen fixing symbiosis. Soybeans are the major source of…

Implications of U(IV)-silicate core-shell colloids for radionuclide transport

Lead Institution

University of Manchester

Principal Investigator

Samuel Shaw

Project type

Large-Scale EMSL Research

Here we will use a range of techniques to advance fundamental and predictive understanding of uranium colloids in the subsurface environment. Recent work has highlighted that U(IV)-silicate colloids…

Characterizing the functional transition of endophytic fungi during plant senescence

Lead Institution

University of California, Santa Barbara

Principal Investigator

Ryoko Oono

Project type

Large-Scale EMSL Research

Decomposition of leaf litter by a diverse microbial community is a major biogeochemical process in the global carbon cycle. Fungi are considered the primary decomposers of plant litter, especially of…

Wet Non-Aqueous Supercritical Fluid Interactions with Densified Model Rock Materials

Lead Institution

St. Mary's College of Maryland

Principal Investigator

Geoffrey Bowers

Project type

Large-Scale EMSL Research

Our research program investigates the fundamental molecular-scale dynamics, energetics, and reactivity of geochemically relevant solid-fluid interfaces by integrating experimental spectroscopy and…

Fluxomics-based elucidation of metabolic remodeling and optimization strategies for enhancing bioproduction efficiency in microbial factories under selection pressure

Lead Institution

University of Maryland, College Park

Principal Investigator

Daniel Dwyer

Project type

Large-Scale EMSL Research

Well characterized bacterial models such as Escherichia coli are ubiquitously used as microbial factories in the commercial production of biofuels and bioproducts. The increasingly optimized…

The persistence of soil organic carbon in the mineralosphere as a function of the soil microbiome, proteome, and exometabolome

Lead Institution

University of California, Berkeley

Principal Investigator

Mary Firestone

Project type

Large-Scale EMSL Research

Soils store more carbon (C) than the atmosphere and biosphere combined, yet the fundamental mechanisms that regulate the persistence of this vast pool of C remain elusive. Soil organic matter (SOM)…

Stabilization of organic carbon by complexation with iron colloids formed during biogeochemical weathering of basalt

Lead Institution

University of Arizona

Principal Investigator

Jon Chorover

Project type

Large-Scale EMSL Research

This project is part of a larger, multi-year effort to evaluate the development of spatial and temporal heterogeneity as a result of microbial colonization and biogeochemical weathering of granular…

Plant Stress in a Changing Environment: Using Plant Metabolomics to Improve Predictions of Biogenic Secondary Organic Aerosol Production

Lead Institution

University of California, Irvine

Principal Investigator

Celia Faiola

Project type

Large-Scale EMSL Research

This research addresses a critical barrier in our understanding of the processes controlling biogenic secondary organic aerosol formation (SOA), and how they are evolving in a changing environment…

Applying multi-dimensional solid state NMR to explore the nanoarchitecture of native and engineered plant cell walls

Lead Institution

University of Adelaide

Principal Investigator

Jennifer Mortimer

Project type

Large-Scale EMSL Research

Plants fix carbon via photosynthesis, the majority of which is incorporated into cell wall polysaccharides. These polysaccharides are synthesized in different cellular compartments, but are assembled…

Towards a metabolic model of circadian clock control of metabolism in Neurospora crassa.

Lead Institution

Rensselaer Polytechnic Institute

Principal Investigator

Jennifer Hurley

Project type

Large-Scale EMSL Research

Biofuel production using cellulases extracted from fungi has the potential to be a major resource to replace fossil fuels and regulating the synthesis of these and other enzymes is crucial for the…

A systems biology approach to identifying the native function of Hg methylation proteins in Desulfovibrio desulfuricans ND132

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

Dwayne Elias

Project type

Large-Scale EMSL Research

Methylmercury (MeHg), an organic Hg compound, is a potent toxin that bioaccumulates in food sources and is primarily produced by microorganisms in anaerobic organic-rich soils and sediments. To date,…

Nano-scale examinations of the sequestration of metal(loid)s by organic matter and mineral surface coatings in soils

Lead Institution

University of Manitoba

Principal Investigator

Michael Schindler

Project type

Large-Scale EMSL Research

Airborne particulate matter (PM) - suspended solid particles with diameters between a few nanometers (nm) and several micrometers - has always been a constituent of the Earth's atmosphere. Man…

Deciphering Lignin-forming Supramolecular Protein Complexes for Facile Biomass Deconstruction

Lead Institution

Washington State University

Principal Investigator

Norman Lewis

Project type

Large-Scale EMSL Research

Our overall aim is to further identify, dissect, and exploit dirigent protein (DP) mediated supramolecular protein complexes for macromolecular lignin assembly in order to ultimately achieve facile…

Quantum Calculations on Ion Channels, Especially Voltage Gated Channels

Lead Institution

City College of New York

Principal Investigator

Michael Green

Project type

Large-Scale EMSL Research

The properties of ion channel proteins, found in essentially all organisms, will be computed using quantum calculations. The principal techniques include optimization at Hartree-Fock level …

Molecular understanding of nutrient sorption on model mineral surfaces

Lead Institution

Oregon State University

Principal Investigator

Kelsey Stoerzinger

Project type

Large-Scale EMSL Research

Iron (Fe)-containing minerals exert a significant influence on soil and sediment geochemistry, such as the cycling of nitrogen (N) and phosphorus (P) nutrients in the bioavailable form of dissolved…

Mechanistic Study of Sulfur Oxidizing Bacteria Using a Combined Experimental and Theoretical Approach: The Role of the SoxB Protein

Lead Institution

Los Alamos National Laboratory

Principal Investigator

Ping Yang

Project type

Large-Scale EMSL Research

The goal of this proposal is to characterize the molecular mechanisms associated to the a Mn2(II,II) core of the SoxB protein. This protein plays a central role in the sulfur oxidizing catalytic…

Oceans as a source of airborne metabolites and primary biological aerosol particles

Lead Institution

State University of New York at Stony Brook

Principal Investigator

Daniel Knopf

Project type

Large-Scale EMSL Research

Ocean waters, which cover 71% of the Earth's surface, contain dissolved organic carbon (DOC) including lipids, humics and organic acids, and free macromolecules, the precursor material for the…

Organic carbon characterization in a warming coastal wetland

Lead Institution

Smithsonian Institution

Principal Investigator

James Megonigal

Project type

Large-Scale EMSL Research

Coastal wetlands are global hotspots of carbon storage, but the future carbon stock stability of coastal wetlands is uncertain as data are lacking on responses to factors such as warming and elevated…