1,000 Fungal Proteins
Campaign name: 1,000 Fungal Proteins
Fungi represent the largest, most diverse group of microorganisms on Earth. With an estimated 1.5 million species, fungi live everywhere from soil to air and in plants, animals, and the human body.
In recent years, the catalog of fungal genome sequences in public databases has grown rapidly. However, functional and structural characterization of the proteins encoded in these sequences has not kept the same pace.
The Environmental Molecular Sciences Laboratory (EMSL) developed the 1,000 Fungal Proteins campaign to address this gap.
The 1,000 Fungal Proteins campaign is designed to associate structure and function for previously uncharacterized proteins that are highly conserved across the fungal kingdom. EMSL will apply experimental and computational resources in structural biology to accelerate the annotation of proteins of unknown function.
Through this campaign, a team of researchers will explore biochemical, genetic, and cell biology approaches to characterize proteins that are nominated or provided by EMSL users.
Samples of interest to 1,000 Fungal Proteins include:
- target protein sequences
- target genes that are cell-free vectored
- target proteins or multi-protein complexes that have been purified
- fungal cells with high-copy of target protein in-vivo
Instruments and resources
1,000 Fungal Proteins-related projects will have access to and use of the following EMSL instrumentation and resources:
- Cell-free expression pipeline
- Native Mass Spectrometry
- Nuclear Magnetic Resonance
- Krios Cryo-Transmission Electron Microscope
- Aquilos 2 Cryo-Focused Ion Beam/Scanning Electron Microscope
- Tahoma mid-range scientific computing
Campaign launch
The scientific community will be encouraged to submit samples at various entry points into a pipeline for the campaign, ranging from users with highly purified proteins or metabolites to those who have a gene sequence and would apply computational or experimental workflows to their research.
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