Doo Nam Kim

Enhancing biopreparedness through a model system to understand the molecular mechanisms that lead to pathogenesis and disease transmission

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Margaret Cheung-Wyker

The science of biopreparedness to counter biological threats hinges on understanding the fundamental principles and molecular mechanisms that lead to pathogenesis and disease transmission. Our vision…

Accurately predict the spatial arrangement of protein complexes from subtomogram images through physics-informed machine learning

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Margaret Cheung-Wyker

Deducing three-dimensional (3D) structures of molecular assemblies from cryo-electron tomography (cryoET) data provides an unprecedented level of detail about biological systems in their native…

Assessment of EUV Atom Probe Tomography for Mass Spectral Analysis of Biomolecules

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Daniel Perea

A Local Electrode Atom Probe (LEAP) Atom utilizing a pulsed, coherent, extreme ultraviolet (EUV) source will be used for the first time for mass-spectral and 3D atomic-scale reconstruction analysis…

Virtual Proteomics (ViP): Integrative modeling of protein assemblies in a cell with constraints from proteomic data and reconstructed images from cryo-electron tomography

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Margaret Cheung-Wyker

The objective of the proposed research is to advance the spatial knowledge of higher-order protein assemblies in a cell by integrating improved image reconstruction from cryo-electron tomography and…

Unraveling New Pathways for Carbon Capture to Enhance Lipid-Biomass Feedstocks

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

Organisms have evolved a number of spatially localized processes to efficiently break down, absorb, and use necessary metabolites. Although progress has been made in identifying the genes that are…

Collaborative Access Team: Pacific Northwest Cryo-EM Center (PNCC)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

PNNL will create and operate the Pacific Northwest Center for Cryo-EM (PNCC), a state-of-the-art national center for cryo-electron microscopy (Cryo-EM) that will rapidly and efficiently collect and…

Joining Systems Biology and Bioimaging on Same Cells

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

We plan to develop a new capability at EMSL for coupling bioimaging and systems biology analysis of the same exact cells. The use of the highly simplified and smallest free-living eukaryote…

Opportunities linking omics and structural biology at PNNL: Excelling at Cryo-EM

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

We were recently funded jointly by EMSL and DOE/BER to purchase, install and operate a new Krios G3i cryo-TEM at EMSL. By funding the system jointly, the new microscope would become available to EMSL…

EMSL Science Overview

Doo Nam Kim

Projects

Enhancing biopreparedness through a model system to understand the molecular mechanisms that lead to pathogenesis and disease transmission

Accurately predict the spatial arrangement of protein complexes from subtomogram images through physics-informed machine learning

Assessment of EUV Atom Probe Tomography for Mass Spectral Analysis of Biomolecules

Virtual Proteomics (ViP): Integrative modeling of protein assemblies in a cell with constraints from proteomic data and reconstructed images from cryo-electron tomography

Unraveling New Pathways for Carbon Capture to Enhance Lipid-Biomass Feedstocks

Collaborative Access Team: Pacific Northwest Cryo-EM Center (PNCC)

Joining Systems Biology and Bioimaging on Same Cells

Opportunities linking omics and structural biology at PNNL: Excelling at Cryo-EM